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Molecular characterization and chromosomal localization of mouse Purα gene
Journal article   Peer reviewed

Molecular characterization and chromosomal localization of mouse Purα gene

Vandhana Muralidharan, Laura Cort, Ellen Meier, Elizabeth P Blankenhorn and Kamel Khalili
Journal of cellular biochemistry, v 77(1)
01 Apr 2000
DOI: https://doi.org/10.1002/(SICI)1097-4644(20000401)77:1<1::AID-JCB1>3.0.CO;2-L
PMID: 10679811
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

5′UTR Purα open reading frame
Purα is a 39‐kDa sequence‐specific single‐stranded DNA/RNA binding protein with the ability to modulate transcription of several genes containing the Pur element in their promoter region. Human and mouse Purα exhibit an extraordinary degree of conservation with only two changes at amino acid residues 49 and 306. A 15‐kb genomic clone encompassing the mouse Purα gene was isolated by screening the mouse genomic library, using a PCR‐amplified fragment from human Purα cDNA. Results from sequencing analysis confirmed the isolated genomic clone to be Purα and not the other members of the Pur family, including Purβ. Characterization of the mouse Purα gene by restriction analysis/Southern blotting and sequencing revealed that the Purα gene contains only one intron within the 5′ UTR and the open reading frame was intact. Using chromosomal markers, the Purα gene was mapped to chromosome 18 in mouse and rat. J. Cell. Biochem. 77:1–5, 2000. © 2000 Wiley‐Liss, Inc.

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Collaboration types
Domestic collaboration
Web of Science research areas
Biochemistry & Molecular Biology
Cell Biology
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