Journal article
Molecular clones of α-tubulin genes of Plasmodium yoelii reveal an unusual feature of the carboxy terminus
Molecular and biochemical parasitology, v 30(2), pp 165-174
1988
PMID: 2459618
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
By using a
Trypanosoma brucei α-tubulin cDNA probe under reduced stringency hybridization conditions, we have isolated two genomic clones that constitute portions of α-tubulin genes of the rodent malarial parasite
Plasmodium yoelii, P. yoelii has two α-tubulin genes, the 3′ portions of which were present in the two clones, PyαT1 and PyαT2, containing 1.3 kb and 6.6 kb
EcoRI fragments respectively. The 1358 bp PyαT1 clone was completely sequenced and found to contain 591 nucleotides of uninterrupted coding sequence with a strong bias for AT-rich codons, starting with codon 254 of a consensus α-tubulin sequence. Numerous attempts to clone 5′ portions of these genes were unsuccessful. A single mRNA of 2.3 kb was recognized by both the clones in the erythrocytic stages of
P. yoelii. A probe constituting the untranslated sequences of PyαT1 also recognized this RNA but failed to hybridize with PyαT2 sequences, indicating that the gene represented by the PyαT1 clone was expressed during the erythrocytic stages. The deduced amino acid sequence of the PyαT1 gene terminates in Tyr-Glu instead of Glu-Tyr observed in α-tubulins of almost all other organisms. The difference observed may have implications for α-tubulin metabolism in malarial parasites.
Metrics
Details
- Title
- Molecular clones of α-tubulin genes of Plasmodium yoelii reveal an unusual feature of the carboxy terminus
- Creators
- Rama AkellaPrema ArasuAkhil B Vaidya
- Publication Details
- Molecular and biochemical parasitology, v 30(2), pp 165-174
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:A1988P618800007
- Scopus ID
- 2-s2.0-0023809305
- Other Identifier
- 991014877953304721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Parasitology