Journal article
Molecular hybridization with RNA probes in concentrated solutions of guanidine thiocyanate
Analytical biochemistry, v 163(2)
1987
PMID: 2444134
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Hybridizations with RNA probes were performed in 3–6
m guanidine thiocyanate concentrations where cells can be solubilized. At these concentrations the melting temperature of hybrids and the optimum temperature of hybrid formation are greatly reduced. The rate of hybridization in GuSCN at room temperature was greater than that in 50% formamide at 42°C. Hybridizations were performed on DNA and RNA in cells dissolved in GuSCN without prior nucleic acid purification.
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Details
- Title
- Molecular hybridization with RNA probes in concentrated solutions of guanidine thiocyanate
- Creators
- James Thompson - Hahnemann University HospitalDavid Gillespie - Hahnemann University HospitalJervis Walter Thompson - Digital Media
- Publication Details
- Analytical biochemistry, v 163(2)
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Digital Media
- Web of Science ID
- WOS:A1987H878600001
- Scopus ID
- 2-s2.0-0023351753
- Other Identifier
- 991019184299304721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology
- Chemistry, Analytical