Journal article
Monitoring of ovarian cancer cell invasion in real time with frequency-dependent impedance measurement
American Journal of Physiology: Cell Physiology, v 311(6), pp C1040-C1047
01 Dec 2016
PMID: 27784677
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The conventional approach to assessing cancer invasion is primarily for end-point analysis, which does not provide temporal information on the invasion process or any information on the interactions between invading cells and the underlying adherent cells. To alleviate these limitations, the present study exploited electric cell-substrate impedance sensing (ECIS) to monitor the invasion of ovarian cancer cells (SKOV-3) through an adherent monolayer of human umbilical vein endothelial cells (HUVECs). Impedance was measured at 4 kHz of AC voltage or was measured as a function of AC frequency (25 Hz to 60 kHz). By measuring impedance at 4-kHz AC, we found that the invasion of SKOV-3 cells through the HUVEC monolayer was manifested as a rapid decrease in transendothelial electrical resistance in real time. The invasion was augmented in the presence of hepatocyte growth factor (HGF). The enhancing effect of HGF was attenuated by c-Met inhibitor (SU11274). By measuring the frequency-dependent impedance of SKOV-3 cells over time, we found that HGF-enhanced SKOV-3 cell invasion was accomplished with reduced junctional resistance (Rb), increased average cell-substrate separation (h), and increased micromotion. SU11274 attenuated the effects of HGF on Rb, h, and micromotion in the SKOV-3 monolayer. SU11274 also increased the barrier function of the HUVEC monolayer by increasing Rb and decreasing h. In conclusion, this study demonstrated an improved method for monitoring and studying the interactions between cancer cells and the underlying adherent cells during invasion in real time. Alterations in cellular biophysical properties (Rb, h) associated with cancer transendothelial invasion were detected.
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Details
- Title
- Monitoring of ovarian cancer cell invasion in real time with frequency-dependent impedance measurement
- Creators
- Chun-Min Lo - National Yang Ming UniversityJun-Chih Lo - Nanhua Univ, Grad Inst Nat Healing Sci, Chiayi, TaiwanPriscila Y. Sato - Temple UniversityTsz-Lun Yeung - The University of Texas MD Anderson Cancer CenterSamuel C. Mok - The University of Texas MD Anderson Cancer CenterKay-Pong Yip - University of South Florida
- Publication Details
- American Journal of Physiology: Cell Physiology, v 311(6), pp C1040-C1047
- Publisher
- Amer Physiological Soc
- Number of pages
- 8
- Grant note
- MOST102-2628-B-010-010-MY3 / Ministry of Science and Technology of the Republic of China, Taiwan; Ministry of Science and Technology, Taiwan P30CA008748 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) 1R03-CA-123621-01A1; R01-CA-142832; R01-CA-169200; R01-CA-133057 / National Cancer Institute Grants; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000390110000021
- Scopus ID
- 2-s2.0-85006264052
- Other Identifier
- 991020099666304721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Cell Biology
- Physiology