Journal article
Monoclonal antibody ELISA test indicates that large amounts of constitutive hsp-70 are present in salamanders, turtle and fish
Journal of thermal biology, v 19(1)
1994
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
1.
1. We purified hsp-70 from salamander,
Plethodon cinereus, and established mouse myeloma-B lymphocyte hybridoma cell lines to produce monoclonal antibodies against hsp-70.
2.
2. An ELISA test using the monoclonal antibody allows rapid quantification of hsp-70 from tissue homogenates from large numbers of animals.
3.
3. Salamanders (
P. cinereus, Eurycea bislineata, Desmognathus ochrophaeus, Ambystoma talpoidium), turtle (
Trachemys scripta) and fish (
Fundulus heteroclitus, Pimephales promelas) contain large amounts of constitutive hsp-70 and a small but consistent increase in hsp-70 within 1 h of heat shock.
4.
4. ELISA test indicates that there is considerable variation in hsp-70 level among individuals of a species.
5.
5. Constitutive hsp-70 is present at normal ecological temperatures and additional hsp-70 is induced at temperatures as low as 25°C in
A. talpoideum.
Metrics
Details
- Title
- Monoclonal antibody ELISA test indicates that large amounts of constitutive hsp-70 are present in salamanders, turtle and fish
- Creators
- Zhongmo Yu - Drexel UniversityWayne E. Magee - Drexel UniversityJames R. Spotila - Drexel University
- Publication Details
- Journal of thermal biology, v 19(1)
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:A1994MY31300006
- Scopus ID
- 2-s2.0-0001874380
- Other Identifier
- 991019173900404721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biology
- Zoology