Journal article
Monocyte Culture and Adherence Modifies LPS-Induced IL-6 Production and Inhibition by Hydrocortisone
Clinical immunology and immunopathology, v 72(2), pp 264-272
01 Aug 1994
PMID: 8050200
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The effect of hydrocortisone (HC) on IL-6 production by monocytes (Mo) that were cultured under either adherent or limited-adherent culture conditions was determined. Although freshly isolated Mo produced more IL-6 in response to LPS than Mo cultured 24 hr, culture had little effect on inhibition of IL-6 by HC. In contrast, the conditions of Mo culture had a significant impact on the kinetics of IL-6 production and inhibition by HC. Adherent Mo produced significantly higher levels of IL-6 4 hr after LPS compared to limited-adherent Mo. By 12 hr of LPS stimulation, comparable quantities of IL-6 were produced by both cultures. HC inhibition was complete 4 hr after LPS stimulation of adherent Mo, while 12 hr was required for maximum inhibition of limited-adherent Mo. Production of an IL-6 inhibitor was not responsible for the increased inhibition by HC with adherence, because both IL-6 protein and bioactivity were comparably decreased. The kinetics of inhibition of TNFα and IL-1β production by HC were similarly affected by adherence. Phenotypic analysis of cultured vs freshly isolated Mo showed a decrease in Mo expressing CD14, but not CD11b, which paralleled a decrease in IL-6 production by the cultured Mo. However, there were no phenotypic differences between adherent and limited-adherent Mo. The results demonstrate that the time of Mo culture, as well as adherence, may serve to alter LPS-induced IL-6 production and its inhibition by HC.
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Details
- Title
- Monocyte Culture and Adherence Modifies LPS-Induced IL-6 Production and Inhibition by Hydrocortisone
- Creators
- Lisa Breuninger - Drexel UniversityI.Michael GoonewardeneWalla L. DempseyDonna M. Murasko
- Publication Details
- Clinical immunology and immunopathology, v 72(2), pp 264-272
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:A1994PB32400021
- Scopus ID
- 2-s2.0-0028031557
- Other Identifier
- 991020950449904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Immunology
- Pathology