Journal article
Monomeric sarcosine oxidase: structure of a covalently flavinylated amine oxidizing enzyme
Structure (London), v 7(3), pp 331-345
1999
PMID: 10368302
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Abstract
Background: Monomeric sarcosine oxidases (MSOXs) are among the simplest members of a recently recognized family of eukaryotic and prokaryotic enzymes that catalyze similar oxidative reactions with various secondary or tertiary amino acids and contain covalently bound flavins. Other members of this family include heterotetrameric sarcosine oxidase,
N-methyltryptophan oxidase and pipecolate oxidase. Mammalian sarcosine dehydrogenase and dimethylglycine dehydrogenase may be more distantly related family members.
Results: The X-ray crystal structure of MSOX from
Bacillus sp.
B-0618, expressed in
Escherichia coli, has been solved at 2.0 Å resolution by multiwavelength anomalous dispersion (MAD) from crystals of the selenomethionine-substituted enzyme. Fourteen selenium sites, belonging to two MSOX molecules in the asymmetric unit, were used for MAD phasing and to define the local twofold symmetry axis for electron-density averaging. The structures of the native enzyme and of two enzyme–inhibitor complexes were also determined.
Conclusions: MSOX is a two-domain protein with an overall topology most similar to that of
D-amino acid oxidase, with which it shares 14% sequence identity. The flavin ring is located in a very basic environment, making contact with sidechains of arginine, lysine, histidine and the N-terminal end of a helix dipole. The flavin is covalently attached through an 8
α–S-cysteinyl linkage to Cys315 of the catalytic domain. Covalent attachment is probably self-catalyzed through interactions with the positive sidechains and the helix dipole. Substrate binding is probably stabilized by hydrogen bonds between the substrate carboxylate and two basic sidechains, Arg52 and Lys348, located above the
re face of the flavin ring.
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Details
- Title
- Monomeric sarcosine oxidase: structure of a covalently flavinylated amine oxidizing enzyme
- Creators
- Peter Trickey - Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 S. Euclid Ave, St Louis, MO 63110, USAMary Ann Wagner - Department of Biochemistry, MCP-Hahnemann School of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USAMarilyn Schuman Jorns - Department of Biochemistry, MCP-Hahnemann School of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USA E-mail: jornsm@auhs.eduF Scott Mathews - Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 S. Euclid Ave, St Louis, MO 63110, USA E-mail: mathews@biochem.wustl.edu
- Publication Details
- Structure (London), v 7(3), pp 331-345
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000079411100013
- Scopus ID
- 2-s2.0-0001390566
- Other Identifier
- 991014878365704721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biophysics
- Cell Biology