Journal article
Mouse eosinophil-associated ribonucleases: a unique subfamily expressed during hematopoiesis
Mammalian genome, v 12(5), pp 352-361
May 2001
PMID: 11331942
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Abstract
A unique family of ribonucleases was identified by exhaustive screening of genomic and cDNA libraries using a probe derived from a gene encoding a ribonuclease stored in the mouse eosinophil secondary granule. This family contains at least 13 genes, which encode ribonucleases, and two potential pseudogenes. The conserved sequence identity among these genes (∼70%), as well as the isolation/purification of these ribonucleases from eosinophil secondary granules, has led us to conclude that these genes form a unique clade in the mouse that we have identified as the Ear (Eosinophil-associated ribonuclease) gene family. Analyses of the nucleotide substitutions that have occurred among these ribonuclease genes reveal that duplication events within this family have been episodic, occurring within three unique periods during the past 18 × 106 years. Moreover, comparisons of non-synonymous (Ka) vs. synonymous (Ks) rates of nucleotide substitution show that although these genes conserve residues necessary for RNase activity, selective evolutionary pressure(s) exist such that acquired amino acid changes appear to be advantageous. The selective advantage of these amino acid changes is currently unclear, but the occurrence of this phenomenon in both the mouse and the human highlights the importance of these changes for Ear and, therefore, eosinophil effector function(s).
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Details
- Title
- Mouse eosinophil-associated ribonucleases: a unique subfamily expressed during hematopoiesis
- Creators
- Stephania A Cormier - Department of Biochemistry & Molecular Biology, Samuel C. Johnson Research Building, Mayo Clinic Scottsdale, 13400 E. Shea Boulevard, Scottsdale, AZ 85259, USA USKirsten A Larson - Department of Biochemistry & Molecular Biology, Samuel C. Johnson Research Building, Mayo Clinic Scottsdale, 13400 E. Shea Boulevard, Scottsdale, AZ 85259, USA USShubing Yuan - Department of Biochemistry & Molecular Biology, Samuel C. Johnson Research Building, Mayo Clinic Scottsdale, 13400 E. Shea Boulevard, Scottsdale, AZ 85259, USA USTrella L Mitchell - Department of Biochemistry & Molecular Biology, Samuel C. Johnson Research Building, Mayo Clinic Scottsdale, 13400 E. Shea Boulevard, Scottsdale, AZ 85259, USA USKari Lindenberger - Department of Biochemistry & Molecular Biology, Samuel C. Johnson Research Building, Mayo Clinic Scottsdale, 13400 E. Shea Boulevard, Scottsdale, AZ 85259, USA USPatricia Carrigan - Department of Biochemistry & Molecular Biology, Samuel C. Johnson Research Building, Mayo Clinic Scottsdale, 13400 E. Shea Boulevard, Scottsdale, AZ 85259, USA USNancy A Lee - Department of Biochemistry & Molecular Biology, Samuel C. Johnson Research Building, Mayo Clinic Scottsdale, 13400 E. Shea Boulevard, Scottsdale, AZ 85259, USA USJames J Lee - Department of Biochemistry & Molecular Biology, Samuel C. Johnson Research Building, Mayo Clinic Scottsdale, 13400 E. Shea Boulevard, Scottsdale, AZ 85259, USA US
- Publication Details
- Mammalian genome, v 12(5), pp 352-361
- Publisher
- Springer-Verlag; Berlin/Heidelberg
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000168571100004
- Scopus ID
- 2-s2.0-0035014556
- Other Identifier
- 991014878582504721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biotechnology & Applied Microbiology
- Genetics & Heredity