Journal article
Multi-modal detection of colon malignancy by NIR-tagged recognition polymers and ultrasound contrast agents
International journal of pharmaceutics, v 478(2), pp 504-516
30 Jan 2015
PMID: 25437110
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
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To increase colonoscopy capability to discriminate benign from malignant polyps, we suggest combining two imaging approaches based on targeted polymeric platforms. Water-soluble cationized polyacrylamide (CPAA) was tagged with the near infrared (NIR) dye IR-783-S-Ph-COOH to form Flu–CPAA. The recognition peptide VRPMPLQ (reported to bind specifically to CRC tissues) was then conjugated with the Flu–CPAA to form Flu–CPAA–Pep which was then incorporated into echogenic microbubbles (MBs) made of polylactic acid (PLA) that are highly responsive to ultrasound. The ultimate design includes intravenous administration combined with local ultrasound and intra-colon inspection at the NIR range. In this proof of principle study PLA MBs were prepared by the double emulsion technique and loaded with several types of Flu–CPAA–Pep polymers. After insonation the submicron PLA fragments (SPF)-containing Flu–CPAA–Pep were examined in vitro for their ability to attach to colon cancer cells and in vivo (DMH induced rat model) for their ability to attach to colon malignant tissues and compared to the specific attachment of the free Flu–CPAA–Pep.
The generation of SPF-containing Flu–CPAA–Pep resulted in a tissue attachment similar to that of the free, unloaded Flu–CPAA–Pep. The addition of VRPMPLQ to the polymeric backbone of the Flu–CPAA reduced cytotoxicity and improved the specific binding.
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Details
- Title
- Multi-modal detection of colon malignancy by NIR-tagged recognition polymers and ultrasound contrast agents
- Creators
- Meital Bloch - Hebrew University of JerusalemLauren Jablonowski - Drexel UniversityEylon Yavin - Hebrew University of JerusalemDorit Moradov - Hebrew University of JerusalemIrena Djavsarov - Hebrew University of JerusalemAbraham Nyska - Timrat 36576, Israel.Margaret Wheatley - Drexel UniversityAbraham Rubinstein - Hebrew University of Jerusalem
- Publication Details
- International journal of pharmaceutics, v 478(2), pp 504-516
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000349723300009
- Scopus ID
- 2-s2.0-84916899085
- Other Identifier
- 991019168889604721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Pharmacology & Pharmacy