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Journal article
Multicolor Super-Resolution DNA Imaging for Genetic Analysis
Nano letters, v 12(7), pp 3861-3866
01 Jul 2012
PMID: 22698062
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Many types of cancer and neurodegenerative diseases are caused by abnormalities and variations in the genome. We have designed a high-resolution imaging technique with high throughput and low cost for determining structural variations of genes related to genetic diseases. We initially mapped all seven nicking sites of Nb.BbvCI endonuclease enzyme on lambda DNA. Then we resolved densely labeled patterns of 107 nicking sites on human BAC DNA that is digested by Nb.BsmI and Nb.BbvCI endonuclease enzymes. This high density resulted in several dyes being closer together than the diffraction limit. Overall, detailed DNA nicking sites mapping with 100bp resolution was achieved, which has the potential to reveal information about genetic variance and to facilitate medical diagnosis of several genetic diseases.
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Details
- Title
- Multicolor Super-Resolution DNA Imaging for Genetic Analysis
- Creators
- Murat Baday - University of Illinois at Urbana-ChampaignAaron Cravens - University of Illinois at Urbana-ChampaignAlex Hastie - BioNano Genomics (United States)HyeongJun Kim - University of Illinois at Urbana-ChampaignDeren E. Kudeki - University of Illinois at Urbana-ChampaignPui-Yan Kwok - University of California, San FranciscoMing Xiao - BioNano Genomics (United States)Paul R. Selvin - University of Illinois at Urbana-Champaign
- Publication Details
- Nano letters, v 12(7), pp 3861-3866
- Publisher
- American Chemical Society; Washington, DC
- Number of pages
- 6
- Grant note
- 1063188 / Direct For Biological Sciences; National Science Foundation (NSF); NSF - Directorate for Biological Sciences (BIO) Network for Computational Nanotechnology at Illinois DBI-02-15869; 082265 / NSF; National Science Foundation (NSF) 068625; R01-HG005946 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01HG005946 / NATIONAL HUMAN GENOME RESEARCH INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Human Genome Research Institute (NHGRI)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000306296200085
- Scopus ID
- 2-s2.0-84863847010
- Other Identifier
- 991019167900104721
UN Sustainable Development Goals (SDGs)
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Chemistry, Multidisciplinary
- Chemistry, Physical
- Materials Science, Multidisciplinary
- Nanoscience & Nanotechnology
- Physics, Applied
- Physics, Condensed Matter