Journal article
Multimodal Mass Spectrometry Imaging of N-Glycans and Proteins from the Same Tissue Section
Analytical chemistry (Washington), v 88(15), pp 7745-7753
02 Aug 2016
PMID: 27373711
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
On-tissue digestion matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) can be used to record spatially correlated molecular information from formalin-fixed, paraffin-embedded (FFPE) tissue sections. In this work, we present the in situ multimodal analysis of N-linked glycans and proteins from the same FFPE tissue section. The robustness and applicability of the method are demonstrated for several tumors, including epithelial and mesenchymal tumor types. Major analytical aspects, such as lateral diffusion of the analyte molecules and differences in measurement sensitivity due to the additional sample preparation methods, have been investigated for both N-glycans and proteolytic peptides. By combining the MSI approach with extract analysis, we were also able to assess which mass spectral peaks generated by MALDI-MSI could be assigned to unique N-glycan and peptide identities.
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Details
- Title
- Multimodal Mass Spectrometry Imaging of N-Glycans and Proteins from the Same Tissue Section
- Creators
- Bram Heijs - Leiden University Medical CenterStephanie Holst - Leiden University Medical CenterInge H. Briaire-de Bruijn - Leiden UniversityGabi W. van Pelt - Leiden University Medical CenterArnoud H. de Ru - Leiden University Medical CenterPeter A. van Veelen - Leiden University Medical CenterRichard R. Drake - Medical University of South CarolinaAnand S. Mehta - Drexel UniversityWilma E. Mesker - Leiden University Medical CenterRob A. Tollenaar - Leiden UniversityJudith V. M. G. Bovee - Leiden University Medical CenterManfred Wuhrer - Leiden University Medical CenterLiam A. McDonnell - Leiden University Medical Center
- Publication Details
- Analytical chemistry (Washington), v 88(15), pp 7745-7753
- Publisher
- American Chemical Society; Washington, DC
- Number of pages
- 9
- Grant note
- R01 CA120206; U01 CA168856 / National Cancer Institute (NCI); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) 278535 / European Union Seventh Framework Programmes HighGlycan project R21CA186799 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) Hepatitis B Foundation 93512002 / ZonMW Zenith project "Imaging Mass Spectrometry-Based Molecular Histology: Differentiation and Characterization of Clinically Challenging Soft Tissue Sarcomas" state of South Carolina SmartState Endowed Research program R21CA186799 / National Institutes of Health/National Cancer Institute; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI)
- Resource Type
- Journal article
- Language
- English
- Web of Science ID
- WOS:000380967800045
- Scopus ID
- 2-s2.0-84982746161
- Other Identifier
- 991019319087004721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Chemistry, Analytical