Journal article
Multiple Linked Quantitative Trait Loci within the Tmevd2/Eae3 Interval Control the Severity of Experimental Allergic Encephalomyelitis in DBA/2J Mice
Genes and immunity, v 11(8), pp 649-659
Dec 2010
PMID: 20861860
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Theiler’s murine encephalomyelitis virus-induced demyelination (TMEVD) and experimental allergic encephalomyelitis (EAE) are the principal animal models of multiple sclerosis (MS). Previously we provided evidence that
Tmevd2
and
Eae3
may represent either a shared susceptibility locus or members of a gene complex controlling susceptibility to CNS inflammatory demyelinating disease. To explore the genetic relationship between
Tmevd2
and
Eae3
, we generated a D2.C-
Tmevd2
interval-specific congenic (ISC) line and three overlapping interval-specific recombinant congenic (ISRC) lines in which the
Tmevd2
resistant allele from BALB/cByJ was introgressed onto the TMEVD-susceptible DBA/2J background. These mice, all
H2
d
, were studied for susceptibility to EAE elicited by immunization with PLP
180–199
. Compared to DBA/2J mice, D2.C-
Tmevd2
mice developed a significantly less severe clinical disease course and EAE pathology in the spinal cord, confirming the existence of
Eae3
and its linkage to
Tmevd2
in this strain combination. Compare to DBA/2J and D2.C-
Tmevd2
, all three ISRC lines exhibited clinical disease courses of intermediate severity. Neither differences in
ex vivo
antigen-specific cytokine nor proliferative responses uniquely cosegregated with differences in disease severity. These results indicate that multiple QTL within the
Tmevd2
/
Eae3
interval influence EAE severity, one of which includes a homology region for a QTL found in MS by admixture mapping.
Metrics
Details
- Title
- Multiple Linked Quantitative Trait Loci within the Tmevd2/Eae3 Interval Control the Severity of Experimental Allergic Encephalomyelitis in DBA/2J Mice
- Creators
- Karen M Spach - Department of Medicine, University of Vermont, Burlington, VT 05405Laure K Case - Department of Medicine, University of Vermont, Burlington, VT 05405Rajkumar Noubade - Department of Medicine, University of Vermont, Burlington, VT 05405Chase B Petersen - Department of Medicine, University of Vermont, Burlington, VT 05405Ben McElvany - Department of Medicine, University of Vermont, Burlington, VT 05405Nathan Zalik - Department of Medicine, University of Vermont, Burlington, VT 05405William F Hickey - Department of Pathology, Dartmouth Medical School, Hanover, NH, USA, 03755Elizabeth P Blankenhorn - Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA 19129Cory Teuscher - Department of Medicine, University of Vermont, Burlington, VT 05405
- Publication Details
- Genes and immunity, v 11(8), pp 649-659
- Publisher
- Springer Nature
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:000284877100006
- Scopus ID
- 2-s2.0-78649704898
- Other Identifier
- 991014877691404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Genetics & Heredity
- Immunology