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Multiple deoxyribonuclease activities in nuclei of HeLa cells
Journal article   Peer reviewed

Multiple deoxyribonuclease activities in nuclei of HeLa cells

Judy R. Churchill, Jan Urbanczyk and George P. Studzinski
Biochemical and biophysical research communications, v 53(3), pp 1009-1016
06 Aug 1973
PMID: 4738712

Abstract

Extracts of purified HeLa nuclei contain DNase activities which can be separated by CM-Sephadex column chromatography into four distinct enzymes. These DNases differ in their resistance to thermal inactivation and relative activity on different substrates. Each of these DNases can increase the template-primer activity of native DNA for DNA polymerase action.

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