Journal article
Multipotential Marrow Stromal Cells Transduced to Produce L-DOPA: Engraftment in a Rat Model of Parkinson Disease
Human gene therapy, v 10(15), pp 2539-2549
10 Oct 1999
PMID: 10543618
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Bone marrow stromal cells can be used as an alternative source of cells for neural transplantation and repair. Here, the efficacy of genetically modified marrow stromal cells was examined in a rat model
of Parkinson disease. Rat marrow stromal cells (rMSCs) and human marrow stromal cells (hMSCs) were genetically engineered by transduction with retroviruses encoding tyrosine hydroxylase (TH) and GTP cyclohydrolase
I, the enzyme necessary for production of the tetrahydrobiopterin cofactor for TH (BH
4
). Transduced cells synthesized 3,4-dihydroxyphenylalanine (
L
-DOPA) in vitro and maintained their
multipotentiality after retroviral transduction. To examine the cells in vivo, transduced rMSCs were injected into the striatum of 6-hydroxydopamine-lesioned rats.
L
-DOPA and metabolites were
detected by microdialysis in the denervated striatum of rats that received doubly transduced rMSCs. Also, there was a significant reduction in apomorphine-induced rotation when compared with controls. The
cells engrafted and survived for at least 87 days. However, expression of the transgenes ceased at about 9 days, an observation consistent with reports from other laboratories in which similar retroviruses
were used to express transgenes in the brain.
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Details
- Title
- Multipotential Marrow Stromal Cells Transduced to Produce L-DOPA: Engraftment in a Rat Model of Parkinson Disease
- Creators
- Emily J. Schwarz - Hahnemann University HospitalGuillermo M. AlexanderDarwin J. ProckopS. Ausim Azizi
- Publication Details
- Human gene therapy, v 10(15), pp 2539-2549
- Publisher
- Mary Ann Liebert, Inc
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:000083252500010
- Scopus ID
- 2-s2.0-0033544028
- Other Identifier
- 991019169551404721
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Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Genetics & Heredity
- Medicine, Research & Experimental