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Mutations in apoptotic genes and micronucleus occurrence in vinyl chloride-exposed workers in China
Journal article   Peer reviewed

Mutations in apoptotic genes and micronucleus occurrence in vinyl chloride-exposed workers in China

Nannan Feng, Guoqiao Zheng, Yanhui Hao, Yong Li, Yandan Xu, Xiaowen Xu, Guanghui Zhang, Jinwei Wang, Yongliang Li, Paul Brandt-Rauf, …
Environmental and molecular mutagenesis, v 58(1)
Jan 2017
PMID: 27801956

Abstract

Adult Apoptosis Regulatory Proteins - genetics bcl-2-Associated X Protein - genetics Female Humans Male Micronuclei, Chromosome-Defective - chemically induced Micronuclei, Chromosome-Defective - statistics & numerical data Micronucleus Tests Middle Aged Multivariate Analysis Mutagens - toxicity Occupational Exposure - adverse effects Occupational Exposure - analysis Poisson Distribution Polymorphism, Restriction Fragment Length Polymorphism, Single Nucleotide Proto-Oncogene Proteins c-bcl-2 - genetics Proto-Oncogene Proteins c-mdm2 - genetics Surveys and Questionnaires Tumor Suppressor Protein p53 - genetics Vinyl Chloride - toxicity Young Adult
Vinyl chloride is an occupational carcinogen which caused micronuclei in human directly. It has recently been demonstrated that micronuclei formation could generate a spectrum of genomic rearrangements and play a key role in the early tumorigenesis process. We aimed to investigate the association between polymorphisms in the apoptosis process related genes and micronuclei rate in vinyl chloride-exposed workers in China. Cytokinesis block micronucleus test was performed on 342 vinyl chloride-exposed workers and 107 nonexposed workers to determine chromosomal damage. The polymerase chain reaction and restriction fragment length polymorphism technique were used to detect nine Single Nucleotide Polymorphisms in the apoptosis process related genes. There was a highly significant dose-response relationship between vinyl chloride exposure and chromosomal damage. Individuals carrying the variant heterozygote MDM2 -309T > G (rs2279744) and variant homozygote BCL2 -938C > A (rs2279115) were at higher risk for chromosomal damage compared with their wild-type genotype, respectively. Although individuals possessing the variant genotype of BAX -248G > A (rs4645878) had decreased risk compared with the corresponding wild type, this did not reach statistical significant. Genetic polymorphisms in genes related to apoptosis process may have an impact on chromosomal damage induced by vinyl chloride. Environ. Mol. Mutagen. 58:39-45, 2017. © 2016 Wiley Periodicals, Inc.

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Domestic collaboration
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Web of Science research areas
Environmental Sciences
Genetics & Heredity
Toxicology
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