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Journal article
Myosin II governs intracellular pressure and traction by distinct tropomyosin-dependent mechanisms
Molecular biology of the cell, v 30(10), pp 1170-1181
01 May 2019
PMID: 30865560
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Two-dimensional (2D) substrate rigidity promotes myosin II activity to increase traction force in a process negatively regulated by tropomyosin (Tpm) 2.1. We recently discovered that actomyosin contractility can increase intracellular pressure and switch tumor cells from low-pressure lamellipodia to high-pressure lobopodial protrusions during threedimensional (3D) migration. However, it remains unclear whether these myosin II-generated cellular forces are produced simultaneously, and by the same molecular machinery. Here we identify Tpm 1.6 as a positive regulator of intracellular pressure and confirm that Tpm 2.1 is a negative regulator of traction force. We find that Tpm 1.6 and 2.1 can control intracellular pressure and traction independently, suggesting these myosin II-dependent forces are generated by distinct mechanisms. Further, these tropomyosin-regulated mechanisms can be integrated to control complex cell behaviors on 2D and in 3D environments.
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Details
- Title
- Myosin II governs intracellular pressure and traction by distinct tropomyosin-dependent mechanisms
- Creators
- Kimheak Sao - Drexel UniversityTia M. Jones - Drexel UniversityAndrew D. Doyle - National Institute of Dental and Craniofacial ResearchDebonil Maity - Johns Hopkins UniversityGalina Schevzov - UNSW SydneyYun Chen - Johns Hopkins UniversityPeter W. Gunning - UNSW SydneyRyan J. Petrie - Drexel University
- Publication Details
- Molecular biology of the cell, v 30(10), pp 1170-1181
- Publisher
- Amer Soc Cell Biology
- Number of pages
- 12
- Grant note
- R01GM126054 / National Institute of General Medical Sciences of the National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) R01GM126054 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000466151300005
- Scopus ID
- 2-s2.0-85065253024
- Other Identifier
- 991019167720104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Cell Biology