Journal article
N-BLR, a primate-specific non-coding transcript leads to colorectal cancer invasion and migration
Genome biology, v 18(1), pp 98-98
24 May 2017
PMID: 28535802
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Non-coding RNAs have been drawing increasing attention in recent years as functional data suggest that they play important roles in key cellular processes. N-BLR is a primate-specific long non-coding RNA that modulates the epithelial-to-mesenchymal transition, facilitates cell migration, and increases colorectal cancer invasion.
We performed multivariate analyses of data from two independent cohorts of colorectal cancer patients and show that the abundance of N-BLR is associated with tumor stage, invasion potential, and overall patient survival. Through in vitro and in vivo experiments we found that N-BLR facilitates migration primarily via crosstalk with E-cadherin and ZEB1. We showed that this crosstalk is mediated by a pyknon, a short ~20 nucleotide-long DNA motif contained in the N-BLR transcript and is targeted by members of the miR-200 family. In light of these findings, we used a microarray to investigate the expression patterns of other pyknon-containing genomic loci. We found multiple such loci that are differentially transcribed between healthy and diseased tissues in colorectal cancer and chronic lymphocytic leukemia. Moreover, we identified several new loci whose expression correlates with the colorectal cancer patients' overall survival.
The primate-specific N-BLR is a novel molecular contributor to the complex mechanisms that underlie metastasis in colorectal cancer and a potential novel biomarker for this disease. The presence of a functional pyknon within N-BLR and the related finding that many more pyknon-containing genomic loci in the human genome exhibit tissue-specific and disease-specific expression suggests the possibility of an alternative class of biomarkers and therapeutic targets that are primate-specific.
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Details
- Title
- N-BLR, a primate-specific non-coding transcript leads to colorectal cancer invasion and migration
- Creators
- Isidore Rigoutsos - Thomas Jefferson UniversitySang Kil Lee - Yonsei UniversitySu Youn Nam - The University of Texas MD Anderson Cancer CenterSimone Anfossi - The University of Texas MD Anderson Cancer CenterBarbara Pasculli - The University of Texas MD Anderson Cancer CenterMartin Pichler - The University of Texas MD Anderson Cancer CenterYi Jing - Thomas Jefferson UniversityCristian Rodriguez-Aguayo - The University of Texas MD Anderson Cancer CenterAristeidis G Telonis - Thomas Jefferson UniversitySimona Rossi - The University of Texas MD Anderson Cancer CenterCristina Ivan - The University of Texas MD Anderson Cancer CenterTina Catela Ivkovic - Department of Molecular Medicine, Ruder Boskovic Institute, Zagreb, CroatiaLinda Fabris - The University of Texas MD Anderson Cancer CenterPeter M Clark - University of PennsylvaniaHui Ling - The University of Texas MD Anderson Cancer CenterMasayoshi Shimizu - The University of Texas MD Anderson Cancer CenterRoxana S Redis - The University of Texas MD Anderson Cancer CenterMaitri Y Shah - The University of Texas MD Anderson Cancer CenterXinna Zhang - The University of Texas MD Anderson Cancer CenterYoshinaga Okugawa - Baylor University Medical CenterEun Jung Jung - Gyeongsang National UniversityAristotelis Tsirigos - New York UniversityLi Huang - The University of Texas MD Anderson Cancer CenterJana Ferdin - The University of Texas MD Anderson Cancer CenterRoberta Gafà - University of FerraraRiccardo Spizzo - The University of Texas MD Anderson Cancer CenterMilena S Nicoloso - The University of Texas MD Anderson Cancer CenterAnurag N Paranjape - The University of Texas MD Anderson Cancer CenterMaryam Shariati - The University of Texas MD Anderson Cancer CenterAida Tiron - Nassau University Medical CenterJen Jen Yeh - University of North Carolina at Chapel HillRaul Teruel-Montoya - The University of Texas MD Anderson Cancer CenterLianchun Xiao - The University of Texas MD Anderson Cancer CenterSonia A Melo - Universidade do PortoDavid Menter - The University of Texas MD Anderson Cancer CenterZhi-Qin Jiang - The University of Texas MD Anderson Cancer CenterElsa R Flores - University of South FloridaMassimo Negrini - University of FerraraAjay Goel - Baylor University Medical CenterMenashe Bar-Eli - The University of Texas MD Anderson Cancer CenterSendurai A Mani - The University of Texas MD Anderson Cancer CenterChang Gong Liu - The University of Texas MD Anderson Cancer CenterGabriel Lopez-Berestein - The University of Texas MD Anderson Cancer CenterIoana Berindan-Neagoe - Iuliu Hațieganu University of Medicine and PharmacyManel Esteller - Institució Catalana de Recerca i Estudis AvançatsScott Kopetz - The University of Texas MD Anderson Cancer CenterGiovanni Lanza - University of FerraraGeorge A Calin - The University of Texas MD Anderson Cancer Center
- Publication Details
- Genome biology, v 18(1), pp 98-98
- Publisher
- Springer BMC
- Grant note
- UH3 TR000943 / NCATS NIH HHS P30 CA016672 / NCI NIH HHS P50 CA093459 / NCI NIH HHS R01 CA155243 / NCI NIH HHS P50 CA127001 / NCI NIH HHS R35 CA197452 / NCI NIH HHS R01 CA140424 / NCI NIH HHS R01 CA182905 / NCI NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Drexel University
- Web of Science ID
- WOS:000401794000001
- Scopus ID
- 2-s2.0-85019835269
- Other Identifier
- 991019356341404721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Genetics & Heredity