Logo image
Back
NADH and flavin fluorescence responses of starved yeast cultures to substrate additions
Journal article   Peer reviewed

NADH and flavin fluorescence responses of starved yeast cultures to substrate additions

S A Siano and R Mutharasan
Biotechnology and bioengineering, v 34(5), pp 660-670
20 Aug 1989
DOI: https://doi.org/10.1002/bit.260340510
PMID: 18588150
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

Model experiments were performed with starved yeast (Saccharomyces cerevisiae) cultures in a batch reactor in order to develop a better understanding of NAD(P)H and flavin culture fluorescence. Fluorescence was monitored during aerobic-anaerobic-aerobic transitions and ethanol and glucose substrate addition experiments. Interpretations of the fluorescence responses obtained are provided, with consideration given to redox compartmentation and the formation of ethanol shortly after a glucose addition. An analytical spectrofluorophotometer was interfaced to a personal computer and adapted to measure fluorescence in a bioreactor. This was achieved by the use of quartz fiber-optic waveguides to convert the right-angle cuvette geometry of the analytical spectrofluorophotometer to an open-ended fluorescence probe geometry, resulting in a flexible culture fluorescence apparatus. Features of the apparatus include variable excitation and emission wavelengths, allowing for detection of NAD(P)H or flavin fluorescence, as well as small slit widths, a variable sampling rate, excitation and emission scanning capabilities, and good sensitivity.

Metrics

12 Record Views
38 citations in Web of Science
40 citations in Scopus

Details

UN Sustainable Development Goals (SDGs)

This publication has contributed to the advancement of the following goals:

#3 Good Health and Well-Being

InCites Highlights

Data related to this publication, from InCites Benchmarking & Analytics tool:

Web of Science research areas
Biotechnology & Applied Microbiology
Logo image