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Journal article
NDEL1 Phosphorylation by Aurora-A Kinase Is Essential for Centrosomal Maturation, Separation, and TACC3 Recruitment
Molecular and cellular biology, v 27(1), pp 352-367
Jan 2007
PMID: 17060449
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Abstract
ABSTRACT
NDEL1 is a binding partner of LIS1 that participates in the regulation of
cytoplasmic dynein function and microtubule organization during mitotic
cell division and neuronal migration. NDEL1 preferentially localizes to
the centrosome and is a likely target for cell cycle-activated kinases,
including CDK1. In particular, NDEL1 phosphorylation by CDK1 facilitates katanin p60 recruitment to the centrosome and triggers microtubule remodeling. Here, we show that Aurora-A phosphorylates NDEL1 at Ser251 at the beginning of mitotic entry.
Interestingly, NDEL1 phosphorylated by Aurora-A was rapidly downregulated thereafter by ubiquitination-mediated protein degradation. In addition, NDEL1 is required for centrosome targeting of TACC3 through the interaction with TACC3. The expression of Aurora-A phosphorylation-mimetic mutants of NDEL1 efficiently rescued the
defects of centrosomal maturation and separation which are
characteristic of Aurora-A-depleted cells. Our findings suggest that
Aurora-A-mediated phosphorylation of NDEL1 is essential for centrosomal
separation and centrosomal maturation and for mitotic
entry.
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Details
- Title
- NDEL1 Phosphorylation by Aurora-A Kinase Is Essential for Centrosomal Maturation, Separation, and TACC3 Recruitment
- Creators
- Daisuke Mori - Osaka City UniversityYoshihisa Yano - Osaka City UniversityKazuhito Toyo-oka - Osaka City UniversityNoriyuki Yoshida - Osaka City UniversityMasami Yamada - Osaka City UniversityMasami Muramatsu - Saitama Medical UniversityDongwei Zhang - Kumamoto UniversityHideyuki Saya - Kumamoto UniversityYoko Y. Toyoshima - The University of TokyoKazuhisa Kinoshita - Max Planck Institute of Molecular Cell Biology and GeneticsAnthony Wynshaw-Boris - University of California San DiegoShinji Hirotsune - Osaka City University
- Publication Details
- Molecular and cellular biology, v 27(1), pp 352-367
- Publisher
- American Society for Microbiology (ASM)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000243136800028
- Scopus ID
- 2-s2.0-33845796538
- Other Identifier
- 991020100190504721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology