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NLRP3 Inflammasome Is a Target for Development of Broad-Spectrum Anti-Infective Drugs
Journal article   Open access   Peer reviewed

NLRP3 Inflammasome Is a Target for Development of Broad-Spectrum Anti-Infective Drugs

James D Thacker, Brian J Balin, Denah M Appelt, Sihem Sassi-Gaha, Mitali Purohit, Richard F Rest and Carol M Artlett
Antimicrobial agents and chemotherapy, v 56(4), pp 1921-1930
Apr 2012
PMID: 22290938
url
https://doi.org/10.1128/AAC.06372-11View
Published, Version of Record (VoR) Open

Abstract

Experimental Therapeutics
We describe the molecular mode of action and pharmacodynamics of a new molecular entity (NME) that induces the NLRP3 inflammasome-mediated innate immune response. This innate response reduces the pathogen load in an experimentally induced methicillin-resistant Staphylococcos aureus infection, enhances survival in an experimentally induced Gram-negative bacteremia, and overrides the escape mechanism of an obligate intracellular pathogen, viz . Chlamydia pneumoniae . Furthermore, the NME is more effective than standard-of-care antibiotic therapy in a clinically established multifactorial bacterial infection. Analysis of transcriptional regulation of inflammasome signaling genes and innate/adaptive immune genes revealed consistent and significant host changes responsible for the improved outcomes in these infections. These studies pave the way for the development of first-in-class drugs that enhance inflammasome-mediated pathogen clearance and identify the NLRP3 inflammasome as a drug target to address the global problem of emerging new infectious diseases and the reemergence of old diseases in an antibiotic-resistant form.

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Collaboration types
Domestic collaboration
Web of Science research areas
Microbiology
Pharmacology & Pharmacy
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