Journal article
NMDA receptor subunit expression in GABAergic interneurons in the prefrontal cortex: Application of laser microdissection technique
Journal of neuroscience methods, v 176(2), pp 172-181
2009
PMID: 18845188
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The selective involvement of a subset of neurons in many psychiatric disorders, such as gamma-aminobutyric acid (GABA)-ergic interneurons in schizophrenia, creates a significant need for in-depth analysis of these cells. Here we introduce a combination of techniques to examine the relative gene expression of N-methyl-
d-aspartic acid (NMDA) receptor subtypes in GABAergic interneurons from the rat prefrontal cortex. Neurons were identified by immunostaining, isolated by laser microdissection and RNA was prepared for reverse transcription polymerase chain reaction (RT-PCR) and real-time PCR. These experimental procedures have been described individually; however, we found that this combination of techniques is powerful for the analysis of gene expression in individual identified neurons. This approach provides the means to analyze relevant molecular mechanisms that are involved in the neuropathological process of a devastating brain disorder.
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Details
- Title
- NMDA receptor subunit expression in GABAergic interneurons in the prefrontal cortex: Application of laser microdissection technique
- Creators
- Dong Xi - Department of Neurobiology and Anatomy, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, United StatesBenjamin Keeler - Department of Neurobiology and Anatomy, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, United StatesWentong Zhang - Department of Pediatric Surgery, Qilu Hospital and College of Medicine, Shandong University, 250012, ChinaJohn D Houle - Department of Neurobiology and Anatomy, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, United StatesWen-Jun Gao - Department of Neurobiology and Anatomy, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, United States
- Publication Details
- Journal of neuroscience methods, v 176(2), pp 172-181
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000262758100017
- Scopus ID
- 2-s2.0-57749184338
- Other Identifier
- 991014878247604721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemical Research Methods
- Neurosciences