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NOP2/Sun RNA methyltransferase 2 promotes tumor progression via its interacting partner RPL6 in gallbladder carcinoma
Journal article   Open access   Peer reviewed

NOP2/Sun RNA methyltransferase 2 promotes tumor progression via its interacting partner RPL6 in gallbladder carcinoma

Yuan Gao, Zheng Wang, Yidi Zhu, Qin Zhu, Yang Yang, Yunpeng Jin, Fei Zhang, Lin Jiang, Yuanyuan Ye, Huaifeng Li, …
Cancer science, v 110(11), pp 3510-3519
Nov 2019
PMID: 31487418
url
https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/cas.14190View
Published, Version of Record (VoR) Open
url
https://doi.org/10.1111/cas.14190View
Published, Version of Record (VoR) Open

Abstract

Animals Carcinoma - metabolism Carcinoma - pathology Carcinoma - therapy Cell Line, Tumor Cell Proliferation Cholecystitis - metabolism Disease Progression Gallbladder Neoplasms - metabolism Gallbladder Neoplasms - pathology Gallbladder Neoplasms - therapy Humans Methyltransferases - antagonists & inhibitors Methyltransferases - metabolism Mice Mice, Nude Neoplasm Proteins - metabolism Ribosomal Proteins - metabolism Tumor Stem Cell Assay Up-Regulation
NOP2/Sun domain family, member 2 (NSUN2) is a nuclear RNA methyl-transferase catalyzing 5-methylcytosine formation. Evidence shows that NSUN2 is correlated with cell unlimited proliferation. However, its functional role in gallbladder carcinoma (GBC), which is the most common biliary tract malignancy and has a poor prognosis, remains to be determined. Here we found that NSUN2 was highly expressed in GBC tissues as well as cell lines. NSUN2 silencing repressed GBC cell proliferation and tumorigenesis both in vitro and in vivo. Conversely, upregulation of NSUN2 enhanced GBC cell growth and colony formation. We further discovered that RPL6 was a closely interacting partner with NSUN2. Silencing RPL6 resulted in insufficient NSUN2 translational level and accumulative NSUN2 transcriptional level. Exogenous expression of NSUN2 partially rescued the effect of RPL6 in gallbladder cancer progression. Taken together, our data provided novel mechanic insights into the function of NSUN2 in GBC, thus pointing to NSUN2 as a potential and effective therapeutic approach to GBC treatment.

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Web of Science research areas
Oncology
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