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Accepted (AM)Open Access (License Unspecified), Open
Journal article
NOT Gate Genetic Circuits to Control Gene Expression in Cyanobacteria
ACS synthetic biology, v 6(12), pp 2175-2182
01 Dec 2017
PMID: 28803467
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
To downregulate gene expression in cyanobacteria, we constructed NOT gate genetic circuits using orthogonal promoters and their cognate repressors regulated translationally by synthetic riboswitches. Four NOT gates were tested and characterized in five cyanobacterial strains using fluorescent reporter-gene assays. In comparison to alternative systems used to downregulate gene expression in cyanobacteria, these NOT gates performed well, reducing YFP reporter expression by 4 to 50-fold. We further evaluated these NOT gates by controlling the expression of the ftsZ gene, which encodes a prokaryotic tubulin homologue that is required for cell division and is essential for Synechococcus elongatus PCC 7942. These NOT gates would facilitate cyanobacterial genetic engineering or the study of essential cellular processes.
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Details
- Title
- NOT Gate Genetic Circuits to Control Gene Expression in Cyanobacteria
- Creators
- Arnaud Taton - University of California, San DiegoAmy T. Ma - University of California San DiegoMizuho Ota - University of California, San DiegoSusan S. Golden - University of California, San DiegoJames W. Golden - University of California, San DiegoUniv. of California, San Diego, CA (United States)
- Publication Details
- ACS synthetic biology, v 6(12), pp 2175-2182
- Publisher
- American Chemical Society; Washington, DC
- Number of pages
- 8
- Grant note
- 500-10-039 / California Energy Commission [CILMSF] DE-EE0003373 / Department of Energy; United States Department of Energy (DOE) Life Sciences Research Foundation - Howard Hughes Medical Institute R01GM118815 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000418395600002
- Scopus ID
- 2-s2.0-85038637226
- Other Identifier
- 991019168134004721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods