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Naive Human T Cells Develop into Th1 or Th0 Effectors and Exhibit Cytotoxicity Early after Stimulation with Leishmania-Infected Macrophages
Journal article   Open access   Peer reviewed

Naive Human T Cells Develop into Th1 or Th0 Effectors and Exhibit Cytotoxicity Early after Stimulation with Leishmania-Infected Macrophages

Donna M. Russo, Pampa Chakrabarti and James M. Burns
The Journal of infectious diseases, v 177(5), pp 1345-1351
01 May 1998
PMID: 9593023
url
https://doi.org/10.1086/515284View
Published, Version of Record (VoR) Open

Abstract

Studies of human disease suggest that naturally acquired immunity is the predominant outcome of Leishmania infection. Normally protective immune mechanisms activated during asymptomatic or self-healing infections may be minimal in patients who develop disease. To explore early immune responses, an in vitro model of human Leishmania infection was developed in which naive T cells were sensitized with Leishmania-infected macrophages. An analysis of Leishmania-specific cytokine production by these T cell lines revealed that most individuals developed Th1 or Th0 responses early after infection. Infected macrophages from Th1 responders produced interleukin-12. Th0 responders who produced little or no endogenous interleukin-12 could be converted to the Th1 phenotype by addition of interleukin-12 during priming. Finally, infection-sensitized T cells specifically lysed Leishmania-infected macrophages. Thus, this in vitro model system can be used to delineate protective human immune responses against Leishmania induced early after infection.

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Web of Science research areas
Immunology
Infectious Diseases
Microbiology
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