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Journal article
Nanoallergens: A multivalent platform for studying and evaluating potency of allergen epitopes in cellular degranulation
Experimental biology and medicine (Maywood, N.J.), v 241(9), pp 996-1006
May 2016
PMCID: PMC4950352
PMID: 27188517
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Degranulation caused by type I hypersensitivity (allergies) is a complex biophysical process, and available experimental models for studying relevant immunoglobulin E binding epitopes on allergen proteins lack the ability to adequately evaluate, rank, and associate these epitopes individually and with each other. In this study, we propose a new allergy model system for studying potential allergen epitopes using nanoallergens, liposomes modified to effectively display IgE binding epitopes/haptens. By utilizing the covalently conjugated lipid tails on two hapten molecules (dinitrophenol and dansyl), hapten molecules were successfully incorporated into liposomes with high precision to form nanoallergens. Nanoallergens, with precisely controlled high-particle valency, can trigger degranulation with much greater sensitivity than commonly used bovine serum albumin conjugates. In rat basophil leukemia cell experiments, nanoallergens with only 2% hapten loading were able to trigger degranulation in vitro at concentrations as low as 10 pM. Additionally, unlike bovine serum albumin-hapten conjugates, nanoallergens allow exact control over particle size and valency. By varying the nanoallergen parameters such as size, valency, monovalent affinity of hapten, and specific IgE ratios, we exposed the importance of these variables on degranulation intensity while demonstrating nanoallergens' potential for evaluating both high- and low-affinity epitopes. The data presented in this article establish nanoallergen platform as a reliable and versatile allergy model to study and evaluate allergen epitopes in mast cell degranulation.
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Details
- Title
- Nanoallergens: A multivalent platform for studying and evaluating potency of allergen epitopes in cellular degranulation
- Creators
- Peter E Deak - University of Notre DameMaura R Vrabel - University of Notre DameVincenzo J Pizzuti - University of Notre DameTanyel Kiziltepe - University of Notre DameBasar Bilgicer - University of Notre Dame
- Publication Details
- Experimental biology and medicine (Maywood, N.J.), v 241(9), pp 996-1006
- Publisher
- Sage
- Grant note
- R01 AI108884 / NIAID NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:000376383300013
- Scopus ID
- 2-s2.0-84971624020
- Other Identifier
- 991019520540904721
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- Web of Science research areas
- Medicine, Research & Experimental