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Journal article
Nanobiomechanics of Repair Bone Regenerated by Genetically Modified Mesenchymal Stem Cells
Tissue engineering. Part A, v 14(10), pp 1709-1720
01 Oct 2008
PMID: 18620480
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Genetically modified mesenchymal stem cells (MSCs), overexpressing a BMP gene, have been previously shown to be potent inducers of bone regeneration. However, little was known of the chemical and intrinsic nanomechanical properties of this engineered bone. A previous study utilizing microcomputed tomography, back-scattered electron microscopy, energy-dispersive X-ray, nanoindentation, and atomic force microscopy showed that engineered ectopic bone, although similar in chemical composition and topography, demonstrated an elastic modulus range (14.6-22.1 GPa) that was less than that of the native bone (16.6-38.5 GPa). We hypothesized that these results were obtained due to the specific conditions that exist in an intramuscular ectopic implantation site. Here, we implanted MSCs overexpressing BMP-2 gene in an orthotopic site, a nonunion radial bone defect, in mice. The regenerated bone tissue was analyzed using the same methods previously utilized. The samples revealed high similarity between the engineered and native radii in chemical structure and elemental composition. In contrast to the previous study, nanoindentation data showed that, in general, the native bone exhibited a statistically similar elastic modulus values compared to that of the engineered bone, while the hardness was found to be marginally statistically different at 1000 mu N and statistically similar at 7000 mu N. We hypothesize that external loading, osteogenic cytokines and osteoprogenitors that exist in a fracture site could enhance the maturation of engineered bone derived from BMP-modified MSCs. Further studies should determine whether longer duration periods postimplantation would lead to increased bone adaptation.
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Details
- Title
- Nanobiomechanics of Repair Bone Regenerated by Genetically Modified Mesenchymal Stem Cells
- Creators
- Kuangshin Tai - Massachusetts Institute of TechnologyGadi Pelled - Hebrew University of JerusalemDima Sheyn - Hebrew University of JerusalemAnna Bershteyn - Massachusetts Institute of TechnologyLin HanIlan Kallai - Hebrew University of JerusalemYoram Zilberman - Hebrew University of JerusalemChristine Ortiz - Massachusetts Institute of TechnologyDan Gazit - Hebrew University of Jerusalem
- Publication Details
- Tissue engineering. Part A, v 14(10), pp 1709-1720
- Publisher
- Mary Ann Liebert, Inc
- Number of pages
- 12
- Grant note
- Whitaker Foundation U. S. Army Research Office DAAD-19-02D0002 / MIT Institute for Soldier Nanotechnologies
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000259998000012
- Scopus ID
- 2-s2.0-53049084171
- Other Identifier
- 991019186965104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Cell & Tissue Engineering
- Cell Biology
- Engineering, Biomedical
- Materials Science, Biomaterials