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Nanomechanical phenotype of chondroadherin-null murine articular cartilage
Journal article   Open access   Peer reviewed

Nanomechanical phenotype of chondroadherin-null murine articular cartilage

Michael A. Batista, Hadi T. Nia, Patrik Önnerfjord, Karen A. Cox, Christine Ortiz, Alan J. Grodzinsky, Dick Heinegård and Lin Han
Matrix biology, v 38(Jun-02)
Sep 2014
DOI: https://doi.org/10.1016/j.matbio.2014.05.008
PMID: 24892719
Featured in Collection :   UN Sustainable Development Goals @ Drexel
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https://doi.org/10.1016/j.matbio.2014.05.008View
Published, Version of Record (VoR)CC BY-NC-ND V4.0,  Open

Abstract

Cartilage Chondroadherin Collagen Murine model Nanoindentation
Chondroadherin (CHAD), a class IV small leucine rich proteoglycan/protein (SLRP), was hypothesized to play important roles in regulating chondrocyte signaling and cartilage homeostasis. However, its roles in cartilage development and function are not well understood, and no major osteoarthritis-like phenotype was found in the murine model with CHAD genetically deleted (CHAD−/−). In this study, we used atomic force microscopy (AFM)-based nanoindentation to quantify the effects of CHAD deletion on changes in the biomechanical function of murine cartilage. In comparison to wild-type (WT) mice, CHAD-deletion resulted in a significant ≈70–80% reduction in the indentation modulus, Eind, of the superficial zone knee cartilage of 11weeks, 4months and 1year old animals. This mechanical phenotype correlates well with observed increases in the heterogeneity collagen fibril diameters in the surface zone. The results suggest that CHAD mainly plays a major role in regulating the formation of the collagen fibrillar network during the early skeletal development. In contrast, CHAD-deletion had no appreciable effects on the indentation mechanics of middle/deep zone cartilage, likely due to the dominating role of aggrecan in the middle/deep zone. The presence of significant rate dependence of the indentation stiffness in both WT and CHAD−/− knee cartilage suggested the importance of both fluid flow induced poroelasticity and intrinsic viscoelasticity in murine cartilage biomechanical properties. Furthermore, the marked differences in the nanomechanical behavior of WT versus CHAD−/− cartilage contrasted sharply with the relative absence of overt differences in histological appearance. These observations highlight the sensitivity of nanomechanical tools in evaluating structural and mechanical phenotypes in transgenic mice. •Chondroadherin deletion significantly reduces cartilage superficial layer modulus.•Reduction in modulus persists across murine age from 11weeks to one year.•Collagen fibril diameters exhibit increased heterogeneity upon chondroadherin deletion.•Modulus change is not significant in the middle/deep cross-section of CHAD−/− cartilage.•Chondroadherin likely affects cartilage modulus by regulating collagen network.

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Collaboration types
Domestic collaboration
International collaboration
Web of Science research areas
Biochemistry & Molecular Biology
Cell Biology
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