Journal article
Nanoparticle mechanics: deformation detection via nanopore resistive pulse sensing
Nanoscale, v 8(30), pp 14420-14431
01 Jan 2016
PMID: 27321911
Abstract
Solid-state nanopores have been widely used in the past for single-particle analysis of nanoparticles, liposomes, exosomes and viruses. The shape of soft particles, particularly liposomes with a bilayer membrane, can greatly differ inside the nanopore compared to bulk solution as the electric field inside the nanopores can cause liposome electrodeformation. Such deformations can compromise size measurement and characterization of particles, but are often neglected in nanopore resistive pulse sensing. In this paper, we investigated the deformation of various liposomes inside nanopores. We observed a significant difference in resistive pulse characteristics between soft liposomes and rigid polystyrene nanoparticles especially at higher applied voltages. We used theoretical simulations to demonstrate that the difference can be explained by shape deformation of liposomes as they translocate through the nanopores. Comparing our results with the findings from electrodeformation experiments, we demonstrated that the rigidity of liposomes can be qualitatively compared using resistive pulse characteristics. This application of nanopores can provide new opportunities to study the mechanics at the nanoscale, to investigate properties of great value in fundamental biophysics and cellular mechanobiology, such as virus deformability and fusogenicity, and in applied sciences for designing novel drug/gene delivery systems.
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Details
- Title
- Nanoparticle mechanics: deformation detection via nanopore resistive pulse sensing
- Creators
- Armin Darvish - Drexel UniversityGaurav Goyal - Drexel UniversityRachna Aneja - Drexel UniversityRamalingam V. K. Sundaram - Drexel UniversityKidan Lee - Seoul National UniversityChi Won Ahn - National NanoFab CenterKi-Bum Kim - Seoul National UniversityPetia M. Vlahovska - Brown UniversityMin Jun Kim - Drexel UniversityKeith Russell Lee - Health Management and Policy
- Publication Details
- Nanoscale, v 8(30), pp 14420-14431
- Publisher
- Royal Soc Chemistry
- Number of pages
- 12
- Grant note
- 1435000; 1562505 / National Science Foundation (CMMI); National Science Foundation (NSF) 2012-0009563 / Pioneer Research Center Program through the National Research Foundation of Korea - Ministry of Science, ICT & Future Planning NRF-2015K1A4A3047100; NRF-2015M3A7B6027973 / National Research Foundation of Korea (GRDC) 1712069 / Div Of Civil, Mechanical, & Manufact Inn; National Science Foundation (NSF); NSF - Directorate for Engineering (ENG)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics; Health Management and Policy
- Web of Science ID
- WOS:000381417800011
- Scopus ID
- 2-s2.0-84980039402
- Other Identifier
- 991019173959604721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Chemistry, Multidisciplinary
- Materials Science, Multidisciplinary
- Nanoscience & Nanotechnology
- Physics, Applied