Journal article
Nanoprobe NMR spectroscopy and in vivo microdialysis: new analytical methods to study brain neurochemistry
Journal of neuroscience methods, v 133(1), pp 181-189
15 Feb 2004
PMID: 14757359
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Nuclear magnetic resonance (NMR) spectroscopy was used to study the chemical composition of cerebrospinal fluid (CSF) microdialysate from the rat brain. In vivo microdialysis techniques were used in several brain regions including the frontal cortex, amygdala, striatum, nucleus accumbens and third ventricle and dialysate samples (20
μl) were subsequently analyzed by one and two-dimensional
1
H
NMR experiments using a Varian nanoprobe. Neurochemical resonances were assigned on the basis of published chemical shifts [Lindon et al., Ann. Rep. NMR Spectrosc. 38 (1999) 1–88], correlation experiments and addition of standard compounds. Glucose, lactate, formate, pyruvate, creatinine, γ-hydroxybutyrate, acetate, glutamate, glycine, tyrosine, isoleucine, leucine, alanine and choline were some of the neurochemicals unambiguously assigned. Additional studies in the frontal cortex showed that amino acids such as glutamate, alanine and isoleucine were sensitive to local tetrodotoxin (TTX) infusion. The NMR spectra were also subjected to multivariate statistical methods to compare the different brain regions examined. To our knowledge, the present experiments are the first to describe the combination of nanoprobe NMR technology with in vivo microdialysis for the analysis of brain neurochemistry in freely-moving rats.
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Details
- Title
- Nanoprobe NMR spectroscopy and in vivo microdialysis: new analytical methods to study brain neurochemistry
- Creators
- Purnima Khandelwal - Princeton UniversityChad E. Beyer - Neuroscience Discovery Research, Wyeth Research, CN 8000, Princeton, NJ 08543-8000, USAQian Lin - Neuroscience Discovery Research, Wyeth Research, CN 8000, Princeton, NJ 08543-8000, USAPaul McGonigle - Neuroscience Discovery Research, Wyeth Research, CN 8000, Princeton, NJ 08543-8000, USALee E. Schechter - Neuroscience Discovery Research, Wyeth Research, CN 8000, Princeton, NJ 08543-8000, USAAlvin C. Bach - Chemical and Screening Sciences, Discovery Analytical Chemistry, Wyeth Research, CN 8000, Princeton, NJ 08543-8000, USA
- Publication Details
- Journal of neuroscience methods, v 133(1), pp 181-189
- Publisher
- Elsevier
- Number of pages
- 9
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000188887700021
- Scopus ID
- 2-s2.0-0742272067
- Other Identifier
- 991021902504604721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemical Research Methods
- Neurosciences