Journal article
Nanoscale Phase Separation in DSPC-Cholesterol Systems
Langmuir, v 29(31), pp 9832-9840
06 Aug 2013
PMID: 23876059
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The lipid arrangement of eukaryotic cell membranes has been shown to be heterogeneous, with domains enriched in cholesterol and saturated phospholipids, coexisting with a continuous phase that is enriched in unsaturated phospholipids. While the existence of these domains is well-established, there is still a lack of consensus regarding domain size and the factors influencing it. In this work, we investigate model membranes consisting of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)-1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) cholesterol (Chol) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC, 18:1-16:0) DSPC Chol with a steady-state fluorescence assay and report the influence of phospholipid chain saturation and chain length on domain size. The spectral shifts of 1-myristoyl-2-[12-[(5-dimethylamino-1-naphthalenesulfonyl)amino]-dodecanoyl]-sn-glycero-3-phosphocholine (DAN-PC) and a Forster resonance energy transfer (FRET) assay were used, along with an analytical model, to estimate domain sizes. A region of nanoscale domain existence was observed in both ternary systems; however, the domains formed in the system containing the asymmetric lipid (POPC, 18:1-16:0) were larger than those formed in the diunsaturated lipid (DOPC, 18:1-18:1). This is a new finding, as domains were not previously known to exist in similar POPC-based systems.
Metrics
Details
- Title
- Nanoscale Phase Separation in DSPC-Cholesterol Systems
- Creators
- Angela C. Brown - Drexel UniversitySteven P. Wrenn - Drexel University
- Publication Details
- Langmuir, v 29(31), pp 9832-9840
- Publisher
- American Chemical Society; Washington, DC
- Number of pages
- 9
- Grant note
- R01GM071355 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) 1 R01 GM071355 / National Institutes of Health (NIH); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:000323014200027
- Scopus ID
- 2-s2.0-84881440652
- Other Identifier
- 991019167437904721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Multidisciplinary
- Chemistry, Physical
- Materials Science, Multidisciplinary