Journal article
Nanoscale Structural and Mechanical Properties of Nontypeable Haemophilus influenzae Biofilms
Journal of bacteriology, v 191(8), pp 2512-2520
Apr 2009
PMID: 19218382
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Nontypeable
Haemophilus influenzae
(NTHI) bacteria are commensals in the human nasopharynx, as well as pathogens associated with a spectrum of acute and chronic infections. Two important factors that influence NTHI pathogenicity are their ability to adhere to human tissue and their ability to form biofilms. Extracellular polymeric substances (EPS) and bacterial appendages such as pili critically influence cell adhesion and intercellular cohesion during biofilm formation. Structural components in the outer cell membrane, such as lipopolysaccharides, also play a fundamental role in infection of the host organism. In spite of their importance, these pathogenic factors are not yet well characterized at the nanoscale. Here, atomic force microscopy (AFM) was used in aqueous environments to visualize structural details, including probable Hif-type pili, of live NTHI bacteria at the early stages of biofilm formation. Using single-molecule AFM-based spectroscopy, the molecular elasticities of lipooligosaccharides present on NTHI cell surfaces were analyzed and compared between two strains (PittEE and PittGG) with very different pathogenicity profiles. Furthermore, the stiffness of single cells of both strains was measured and subsequently their turgor pressure was estimated.
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Details
- Title
- Nanoscale Structural and Mechanical Properties of Nontypeable Haemophilus influenzae Biofilms
- Creators
- Fernando Terán Arce - Center for Nanomedicine, Department of Medicine, University of Chicago, Chicago, Illinois 60637Ross Carlson - Center for Nanomedicine, Department of Medicine, University of Chicago, Chicago, Illinois 60637James Monds - Center for Nanomedicine, Department of Medicine, University of Chicago, Chicago, Illinois 60637Richard Veeh - Center for Nanomedicine, Department of Medicine, University of Chicago, Chicago, Illinois 60637Fen Z Hu - Center for Nanomedicine, Department of Medicine, University of Chicago, Chicago, Illinois 60637Philip S Stewart - Center for Nanomedicine, Department of Medicine, University of Chicago, Chicago, Illinois 60637Ratnesh Lal - Center for Nanomedicine, Department of Medicine, University of Chicago, Chicago, Illinois 60637Garth D Ehrlich - Center for Nanomedicine, Department of Medicine, University of Chicago, Chicago, Illinois 60637Recep Avci - Center for Nanomedicine, Department of Medicine, University of Chicago, Chicago, Illinois 60637
- Publication Details
- Journal of bacteriology, v 191(8), pp 2512-2520
- Publisher
- American Society for Microbiology (ASM)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000264752600009
- Scopus ID
- 2-s2.0-65249162729
- Other Identifier
- 991014878013104721
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Microbiology