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Nanosecond-Pulsed DBD Plasma-Generated Reactive Oxygen Species Trigger Immunogenic Cell Death in A549 Lung Carcinoma Cells through Intracellular Oxidative Stress
Journal article   Open access   Peer reviewed

Nanosecond-Pulsed DBD Plasma-Generated Reactive Oxygen Species Trigger Immunogenic Cell Death in A549 Lung Carcinoma Cells through Intracellular Oxidative Stress

Abraham Lin, Billy Truong, Sohil Patel, Nagendra Kaushik, Eun Ha Choi, Gregory Fridman, Alexander Fridman and Vandana Miller
International journal of molecular sciences, v 18(5), p966
03 May 2017
DOI: https://doi.org/10.3390/ijms18050966
PMID: 28467380
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.3390/ijms18050966View
Published, Version of Record (VoR)CC BY V4.0,  Open

Abstract

A549 Cells Adenosine Triphosphate - metabolism Analysis of Variance Antigen-Presenting Cells - metabolism Apoptosis - immunology Calreticulin - metabolism Electric Conductivity Electrodes Humans Immunity Immunotherapy - methods Neoplasms - therapy Oxidative Stress - immunology Plasma Gases - therapeutic use Quartz - chemistry Reactive Oxygen Species - immunology Reactive Oxygen Species - metabolism Ultraviolet Rays
A novel application for non-thermal plasma is the induction of immunogenic cancer cell death for cancer immunotherapy. Cells undergoing immunogenic death emit danger signals which facilitate anti-tumor immune responses. Although pathways leading to immunogenic cell death are not fully understood; oxidative stress is considered to be part of the underlying mechanism. Here; we studied the interaction between dielectric barrier discharge plasma and cancer cells for oxidative stress-mediated immunogenic cell death. We assessed changes to the intracellular oxidative environment after plasma treatment and correlated it to emission of two danger signals: surface-exposed calreticulin and secreted adenosine triphosphate. Plasma-generated reactive oxygen and charged species were recognized as the major effectors of immunogenic cell death. Chemical attenuators of intracellular reactive oxygen species successfully abrogated oxidative stress following plasma treatment and modulated the emission of surface-exposed calreticulin. Secreted danger signals from cells undergoing immunogenic death enhanced the anti-tumor activity of macrophages. This study demonstrated that plasma triggers immunogenic cell death through oxidative stress pathways and highlights its potential development for cancer immunotherapy.

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173 citations in Web of Science
173 citations in Scopus

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UN Sustainable Development Goals (SDGs)

This publication has contributed to the advancement of the following goals:

#3 Good Health and Well-Being

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Collaboration types
Domestic collaboration
International collaboration
Web of Science research areas
Biochemistry & Molecular Biology
Chemistry, Multidisciplinary
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