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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Nanotopography Influences Adhesion, Spreading, and Self-renewal of Human Embryonic Stem Cells
ACS nano, v 6(5), pp 4094-4103
16 Apr 2012
PMID: 22486594
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Human embryonic stem cells (hESCs) have great potentials for future cell-based therapeutics. However, their mechanosensitivity to biophysical signals from the cellular microenvironment is not well characterized. Here we introduced an effective microfabrication strategy for accurate control and patterning of nanoroughness on glass surfaces. Our results demonstrated that nanotopography could provide a potent regulatory signal over different hESC behaviors, including cell morphology, adhesion, proliferation, clonal expansion, and self-renewal. Our results indicated that topological sensing of hESCs might include feedback regulation involving mechanosensory integrin-mediated cell-matrix adhesion, myosin II, and E-cadherin. Our results also demonstrated that cellular responses to nanotopography were cell-type specific and as such, we could generate a spatially segregated co-culture system for hESCs and NIH/3T3 fibroblasts using patterned nanorough glass surfaces.
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Details
- Title
- Nanotopography Influences Adhesion, Spreading, and Self-renewal of Human Embryonic Stem Cells
- Creators
- Weiqiang Chen - Integrated Biosystems and Biomechanics Laboratory, University of Michigan, Ann Arbor, MI 48109, USA Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong Department of Biomedical Engineering, Yale University, New Haven, CT 06511, USA Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USALuis G. Villa-Diaz - Integrated Biosystems and Biomechanics Laboratory, University of Michigan, Ann Arbor, MI 48109, USA Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong Department of Biomedical Engineering, Yale University, New Haven, CT 06511, USA Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USAYubing Sun - Integrated Biosystems and Biomechanics Laboratory, University of Michigan, Ann Arbor, MI 48109, USA Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong Department of Biomedical Engineering, Yale University, New Haven, CT 06511, USA Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USAShinuo Weng - Integrated Biosystems and Biomechanics Laboratory, University of Michigan, Ann Arbor, MI 48109, USA Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong Department of Biomedical Engineering, Yale University, New Haven, CT 06511, USA Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USAJin Koo Kim - Integrated Biosystems and Biomechanics Laboratory, University of Michigan, Ann Arbor, MI 48109, USA Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong Department of Biomedical Engineering, Yale University, New Haven, CT 06511, USA Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USARaymond H. W. Lam - Integrated Biosystems and Biomechanics Laboratory, University of Michigan, Ann Arbor, MI 48109, USA Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong Department of Biomedical Engineering, Yale University, New Haven, CT 06511, USA Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USALin Han - University of MichiganRong Fan - Integrated Biosystems and Biomechanics Laboratory, University of Michigan, Ann Arbor, MI 48109, USA Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong Department of Biomedical Engineering, Yale University, New Haven, CT 06511, USA Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USAPaul H. Krebsbach - Integrated Biosystems and Biomechanics Laboratory, University of Michigan, Ann Arbor, MI 48109, USA Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong Department of Biomedical Engineering, Yale University, New Haven, CT 06511, USA Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USAJianping Fu - Integrated Biosystems and Biomechanics Laboratory, University of Michigan, Ann Arbor, MI 48109, USA Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong Department of Biomedical Engineering, Yale University, New Haven, CT 06511, USA Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
- Publication Details
- ACS nano, v 6(5), pp 4094-4103
- Publisher
- American Chemical Society; Washington, DC
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000304231700049
- Scopus ID
- 2-s2.0-84864661434
- Other Identifier
- 991019187062104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Chemistry, Multidisciplinary
- Chemistry, Physical
- Materials Science, Multidisciplinary
- Nanoscience & Nanotechnology