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Nasopharyngeal microbiome composition associated with Streptococcus pneumoniae colonization suggests a protective role of Corynebacterium in young children
Journal article   Open access   Peer reviewed

Nasopharyngeal microbiome composition associated with Streptococcus pneumoniae colonization suggests a protective role of Corynebacterium in young children

Lei Xu, Joshua Earl and Michael E Pichichero
PloS one, v 16(9), pp e0257207-e0257207
2021
DOI: https://doi.org/10.1371/journal.pone.0257207
PMID: 34529731
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1371/journal.pone.0257207View
Published, Version of Record (VoR)CC BY V4.0,  Open

Abstract

Coculture Techniques Coinfection - microbiology Corynebacterium Corynebacterium Infections - complications Corynebacterium Infections - microbiology Female Humans In Vitro Techniques Infant Male Microbiota Nasopharynx - metabolism Nasopharynx - microbiology Pneumococcal Infections - complications Pneumococcal Infections - microbiology Prospective Studies Respiratory Tract Infections - microbiology Risk Factors RNA, Ribosomal, 16S - metabolism Sequence Analysis, RNA Streptococcus pneumoniae
Streptococcus pneumoniae (Spn) is a leading respiratory tract pathogen that colonizes the nasopharynx (NP) through adhesion to epithelial cells and immune evasion. Spn actively interacts with other microbiota in NP but the nature of these interactions are incompletely understood. Using 16S rRNA gene sequencing, we analyzed the microbiota composition in the NP of children with or without Spn colonization. 96 children were included in the study cohort. 74 NP samples were analyzed when children were 6 months old and 85 NP samples were analyzed when children were 12 months old. We found several genera that correlated negatively or positively with Spn colonization, and some of these correlations appeared to be influenced by daycare attendance or other confounding factors such as upper respiratory infection (URI) or Moraxella colonization. Among these genera, Corynebacterium showed a consistent inverse relationship with Spn colonization with little influence by daycare attendance or other factors. We isolated Corynebacterium propinquum and C. pseudodiphtheriticum and found that both inhibited the growth of Spn serotype 22F strain in vitro.

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15 citations in Web of Science
12 citations in Scopus

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Collaboration types
Domestic collaboration
Web of Science research areas
Microbiology
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