Journal article
Nationwide Surveillance of Pfhrp2 Exon 2 Diversity in Plasmodium falciparum Circulating in Symptomatic Malaria Patients Living in Ghana
The American journal of tropical medicine and hygiene, v 106(6), pp 1660-1666
01 Jun 2022
PMID: 35500587
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Reports of increasing false-negative HRP2-based rapid diagnostic test results across Africa require constant monitoring of factors associated with these false-negative outcomes, as failure of this diagnostic tool will have severe consequences on malaria treatment and control programs. This study characterized the extent of genetic diversity in the Plasmodium falciparum histidine-rich protein 2 (Pfhrp2) gene in P. falciparum isolates from symptomatic malaria patients across the regions of Ghana. Exon 2 of Pfhrp2 was amplified from gDNA using polymerase chain reaction. All Pfhrp2-negative samples were subjected to Pf18S rRNA and Pfmsp2 gene amplifications. The amplified Pfhrp2 exon 2 fragments from clonal samples were sent for commercial Sanger sequencing. The type and number of PfHRP2 repeats, classified based on repeat types previously reported, were estimated from the sequence data and compared among geographical regions. About 81% (2,333/ 2,890) of the original microscopy positive dried blood spot (DBS) samples were available and used in this study. The Pfhrp2 exon 2 amplification was successful in 98.5% (2,297/2,333) of the tested samples, with band size ranging from 400 bp to 1,050 bp. A total of 13 out of the 24 previously reported repeat types were identified among the samples, with three samples lacking both type 2 and type 7 repeat motifs. This study suggested that the genetic diversity of Pfhrp2 exon 2 identified in P. falciparum circulating in symptomatic malaria patients in Ghana is unlikely to influence the sensitivity and specificity of HRP2 RDT-based diagnosis.
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Details
- Title
- Nationwide Surveillance of Pfhrp2 Exon 2 Diversity in Plasmodium falciparum Circulating in Symptomatic Malaria Patients Living in Ghana
- Creators
- Dorcas G. Bredu - University of GhanaGeorge K. Ahadzi - University of Cape CoastDonu Dickson - University of GhanaNana Y. Peprah - Ghana Health ServiceAlexander Asamoah - Ghana Health ServiceGeorge A. Asumah - Ghana Health ServiceBenjamin Abuaku - University of GhanaKwame K. Asare - University of Cape CoastDorcas Obiri-Yeboah - University of Cape CoastColby T. Ford - University of North Carolina at CharlotteEugenia Lo - University of North Carolina at CharlotteKeziah L. Malm - Ghana Health ServiceLinda E. Amoah - University of Ghana
- Publication Details
- The American journal of tropical medicine and hygiene, v 106(6), pp 1660-1666
- Publisher
- Amer Soc Trop Med & Hygiene
- Number of pages
- 7
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000828652200022
- Scopus ID
- 2-s2.0-85148210714
- Other Identifier
- 991022192519704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Public, Environmental & Occupational Health
- Tropical Medicine