Journal article
Near real-time detection of Cryptosporidium parvum oocyst by IgM-functionalized piezoelectric-excited millimeter-sized cantilever biosensor
Biosensors & bioelectronics, v 23(7), pp 1039-1045
2008
PMID: 18054480
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Piezoelectric-excited millimeter-sized cantilever (PEMC) biosensors were fabricated and functionalized with immunoglobulin M (IgM) for the detection of
Cryptosporidium parvum oocyst in a flow configuration at 1
mL/min. The detection of 100, 1000, and 10,000
oocysts/mL was achieved with a positive sensor response in less than 1
min. Bovine serum albumin (BSA) was used as a blocking agent in each experiment and was shown to eliminate non-specific binding. The sensor's resonance frequency response correlates with
C. parvum oocyst concentration logarithmically. The oocyst attachment rate was found to increase by an order of magnitude in increasing concentration from 100 to 10,000
oocysts/mL. The significance of these results is that IgM-functionalized PEMC sensors are highly selective and sensitive to
C. parvum oocyst and therefore, have the potential to accurately identify and quantify
C. parvum oocyst in drinking water.
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Details
- Title
- Near real-time detection of Cryptosporidium parvum oocyst by IgM-functionalized piezoelectric-excited millimeter-sized cantilever biosensor
- Creators
- Gossett A Campbell - Pharmaceutical Development, GlaxoSmithKline, 709 Swedeland Road, PA 19406, United StatesRaj Mutharasan - Chemical and Biological Engineering Department, Drexel University, 3141 Chestnut Street, Philadelphia, PA 19104, United States
- Publication Details
- Biosensors & bioelectronics, v 23(7), pp 1039-1045
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:000253620900017
- Scopus ID
- 2-s2.0-38749129750
- Other Identifier
- 991014877891404721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Biophysics
- Biotechnology & Applied Microbiology
- Chemistry, Analytical
- Electrochemistry
- Nanoscience & Nanotechnology