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Negative transcriptional regulation of PHO81 expression in Saccharomyces cerevisiae
Journal article   Peer reviewed

Negative transcriptional regulation of PHO81 expression in Saccharomyces cerevisiae

Caretha L. Creasy, Dalei Shao and Lawrence W. Bergman
Gene, v 168(1), pp 23-29
1996
DOI: https://doi.org/10.1016/0378-1119(95)00737-7
PMID: 8626060

Abstract

acid phosphatase gene fusion inhibitor promoter Repression yeast
The PH081 gene product functions as an inhibitor of the cyclin-cyclin-dependent kinase pair, Pho80-Pho85, and is required for derepression of acid phosphatase-encoding gene ( PH05) expression. PH081 is the only known regulator of this system whose transcriptional expression is regulated by the level of inorganic phosphate. This effect is mediated by the gene products of the PH04 and PH02 (BAS2, GRF10) genes which act as transcription factors. Fine structural analysis of the PH081 promoter region has revealed the existence of a negative regulatory sequence ( NRS). That is, removal of this element causes an approx. 4-fold increase in PH081 expression. The NRS functions in either orientation, but only when located downstream from the activation sequences. Interestingly, this element shows significant homology to a sequence present in the promoter of the PH08 gene, encoding a phosphate-repressible alkaline phosphatase. As electrophoretic mobility shift assay (EMSA) using whole-cell extracts and a NRS-containing DNA fragment detects a protein which specifically binds to this element.

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