Files and links (1)
Published, Version of Record (VoR)CC BY V4.0, Open
Journal article
Nerve transfer for restoration of lower motor neuron-lesioned bladder function. Part 2: correlation between histological changes and nerve evoked contractions
American journal of physiology. Regulatory, integrative and comparative physiology, v 320(6), pp R897-R915
01 Jun 2021
PMID: 33759573
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We determined the effect of pelvic organ decentralization and reinnervation 1 yr later on urinary bladder histology and function. Nineteen canines underwent decentralization by bilateral transection of all coccygeal and sacral (S) spinal roots, dorsal roots of lumbar (L)7, and hypogastric nerves. After exclusions, eight were reinnervated 12 mo postdecentralization with obturator-to-pelvic and sciatic-to-pudendal nerve transfers, then euthanized 8-12 mo later. Four served as long-term decentralized only animals. Before euthanasia, pelvic or transferred nerves and L1-S3 spinal roots were stimulated and maximum detrusor pressure (MDP) recorded. Bladder specimens were collected for histological and ex vivo smooth muscle contractility studies. Both reinnervated and decentralized animals showed less or denuded urothelium, fewer intramural ganglia, and more inflammation and collagen, than controls, although percent muscle was maintained. In reinnervated animals, pgp9.5+ axon density was higher compared with decentralized animals. Ex vivo smooth muscle contractions in response to KCl correlated positively with submucosal inflam-mation, detrusor muscle thickness, and pgp9.5+ axon density. In vivo, reinnervated animals showed higher MDP after stimulation of L1-L6 roots compared with their transected L7-S3 roots, and reinnervated and decentralized animals showed lower MDP than controls after stimulation of nerves (due likely to fibrotic nerve encapsulation). MDP correlated negatively with detrusor collagen and inflammation, and positively with pgp9.5+ axon density and intramural ganglia numbers. These results demonstrate that bladder function can be improved by transfer of obturator nerves to pelvic nerves at 1 yr after decentralization, although the fibrosis and inflammation that developed were associated with decreased contractile function.
Metrics
Details
- Title
- Nerve transfer for restoration of lower motor neuron-lesioned bladder function. Part 2: correlation between histological changes and nerve evoked contractions
- Creators
- Mary F. Barbe - Temple UniversityCourtney L. Testa - Temple UniversityGeneva E. Cruz - Temple UniversityNagat A. Frara - Temple UniversityEkta Tiwari - Temple UniversityLucas J. Hobson - Temple UniversityBrian S. McIntyre - Drexel Univ, Coll Med, Philadelphia, PA 19104 USADanielle S. Porreca - Temple UniversityDania Giaddui - Temple UniversityAlan S. Braverman - Temple UniversityEmily P. Day - Temple UniversityMamta Amin - Temple Univ, Coll Engn, Dept Elect & Comp Engn, Philadelphia, PA 19122 USAJustin M. Brown - Massachusetts General HospitalMichael Mazzei - College Station Medical CenterMichel A. Pontari - Temple UniversityIda J. Wagner - College Station Medical CenterMichael R. Ruggieri - Shriners Hospitals for Children - Philadelphia
- Publication Details
- American journal of physiology. Regulatory, integrative and comparative physiology, v 320(6), pp R897-R915
- Publisher
- Amer Physiological Soc
- Number of pages
- 19
- Grant note
- R01NS070267 / National Institute of Neurological Disorders and Stroke of the National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Medicine
- Web of Science ID
- WOS:000663519400012
- Scopus ID
- 2-s2.0-85108021147
- Other Identifier
- 991021860756904721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Physiology