Journal article
Neuroepithelial stem cell proliferation requires LIS1 for precise spindle orientation and symmetric division
Cell, v 132(3), pp 474-486
08 Feb 2008
PMID: 18267077
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Mitotic spindle orientation and plane of cleavage in mammals is a determinant of whether division yields progenitor expansion and/or birth of new neurons during radial glial progenitor cell (RGPC) neurogenesis, but its role earlier in neuroepithelial stem cells is poorly understood. Here we report that Lis1 is essential for precise control of mitotic spindle orientation in both neuroepithelial stem cells and radial glial progenitor cells. Controlled gene deletion of Lis1 in vivo in neuroepithelial stem cells, where cleavage is uniformly vertical and symmetrical, provokes rapid apoptosis of those cells, while radial glial progenitors are less affected. Impaired cortical microtubule capture via loss of cortical dynein causes astral and cortical microtubules to be greatly reduced in Lis1-deficient cells. Increased expression of the LIS/dynein binding partner NDEL1 restores cortical microtubule and dynein localization in Lis1-deficient cells. Thus, control of symmetric division, essential for neuroepithelial stem cell proliferation, is mediated through spindle orientation determined via LIS1/NDEL1/dynein-mediated cortical microtubule capture.
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Details
- Title
- Neuroepithelial stem cell proliferation requires LIS1 for precise spindle orientation and symmetric division
- Creators
- Jessica Yingling - Center for Human GeneticsYong Ha Youn - Center for Human GeneticsDawn Darling - Center for Human GeneticsKazuhito Toyo-Oka - Osaka City UniversityTiziano Pramparo - Center for Human GeneticsShinji Hirotsune - Osaka City UniversityAnthony Wynshaw-Boris - Center for Human Genetics
- Publication Details
- Cell, v 132(3), pp 474-486
- Publisher
- Elsevier
- Number of pages
- 13
- Grant note
- R01HD047380 / EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD) R01 NS041310; R01 NS041030-04; P30 NS047101; R01 NS041030-03; R01 NS041030-06; R01 NS041030-01A2; NS041310; R01 NS041030; R01 NS041030-05; R01 NS041030-02 / NINDS NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) R01NS041030 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) R01 HD047380; HD047380; R01 HD047380-02; R01 HD047380-03; R01 HD047380-01A1 / NICHD NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000253180700021
- Scopus ID
- 2-s2.0-38849091345
- Other Identifier
- 991020100084004721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology