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Neuron-specific mRNA complexity responses during hippocampal apoptosis after traumatic brain injury
Journal article   Open access   Peer reviewed

Neuron-specific mRNA complexity responses during hippocampal apoptosis after traumatic brain injury

Paolo G Marciano, Julia Brettschneider, Elisabetta Manduchi, Jason E Davis, Scott Eastman, Ramesh Raghupathi, Kathryn E Saatman, Terence P Speed, Christian J Stoeckert, Jr, James H Eberwine, …
The Journal of neuroscience, v 24(12), pp 2866-2876
24 Mar 2004
DOI: https://doi.org/10.1523/JNEUROSCI.5051-03.2004
PMID: 15044525
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1523/JNEUROSCI.5051-03.2004View
Published, Version of Record (VoR) Open

Abstract

Immunohistochemistry In Situ Nick-End Labeling Neurons - pathology Dentate Gyrus - metabolism Oligonucleotide Array Sequence Analysis Mice, Inbred C57BL RNA, Messenger - genetics Gene Expression Regulation Apoptosis - genetics Male Hippocampus - pathology Son of Sevenless Proteins - biosynthesis Brain Injuries - metabolism RNA, Messenger - metabolism Hippocampus - metabolism Animals Mice Neurons - metabolism PrPC Proteins - biosynthesis Brain Injuries - pathology Dentate Gyrus - pathology Disease Models, Animal
In an effort to understand the complexity of genomic responses within selectively vulnerable regions after experimental brain injury, we examined whether single apoptotic neurons from both the CA3 and dentate differed from those in an uninjured brain. The mRNA from individual active caspase 3(+)/terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling [TUNEL(-)] and active caspase 3(+)/TUNEL(+) pyramidal and granule neurons in brain-injured mice were amplified and compared with those from nonlabeled neurons in uninjured brains. Gene analysis revealed that overall expression of mRNAs increased with activation of caspase 3 and decreased to below uninjured levels with TUNEL reactivity. Cell type specificity of the apoptotic response was observed with both regionally distinct expression of mRNAs and differences in those mRNAs that were maximally regulated. Immunohistochemical analysis for two of the most highly differentially expressed genes (prion and Sos2) demonstrated a correlation between the observed differential gene expression after traumatic brain injury and corresponding protein translation.

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