Journal article
Neuron-specific mRNA complexity responses during hippocampal apoptosis after traumatic brain injury
The Journal of neuroscience, Vol.24(12), pp.2866-2876
24 Mar 2004
PMCID: PMC6729833
PMID: 15044525
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In an effort to understand the complexity of genomic responses within selectively vulnerable regions after experimental brain injury, we examined whether single apoptotic neurons from both the CA3 and dentate differed from those in an uninjured brain. The mRNA from individual active caspase 3(+)/terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling [TUNEL(-)] and active caspase 3(+)/TUNEL(+) pyramidal and granule neurons in brain-injured mice were amplified and compared with those from nonlabeled neurons in uninjured brains. Gene analysis revealed that overall expression of mRNAs increased with activation of caspase 3 and decreased to below uninjured levels with TUNEL reactivity. Cell type specificity of the apoptotic response was observed with both regionally distinct expression of mRNAs and differences in those mRNAs that were maximally regulated. Immunohistochemical analysis for two of the most highly differentially expressed genes (prion and Sos2) demonstrated a correlation between the observed differential gene expression after traumatic brain injury and corresponding protein translation.
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Details
- Title
- Neuron-specific mRNA complexity responses during hippocampal apoptosis after traumatic brain injury
- Creators
- Paolo G Marciano - Departments of Neuroscience, Center for Bioinformatics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USAJulia BrettschneiderElisabetta ManduchiJason E DavisScott EastmanRamesh RaghupathiKathryn E SaatmanTerence P SpeedChristian J Stoeckert, JrJames H EberwineTracy K McIntosh
- Publication Details
- The Journal of neuroscience, Vol.24(12), pp.2866-2876
- Publisher
- Society for Neuroscience; United States
- Grant note
- HG02296 / NHGRI NIH HHS AG 9900 / NIA NIH HHS R01-41699 / PHS HHS P50 NS08803 / NINDS NIH HHS R01-NS40978 / NINDS NIH HHS GM34790 / NIGMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Identifiers
- 991014877835204721
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- Industry collaboration
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- Web of Science research areas
- Neurosciences