Journal article
Neuroprotective properties of RT10, a fraction isolated from Parawixia bistriata spider venom, against excitotoxicity injury in neuron-glia cultures
The journal of venomous animals and toxins including tropical diseases, v 25, pp e148818-e148818
2019
PMID: 31131008
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Glutamate (
Glu), the major excitatory neurotransmitter in the mammalian Central Nervous System (CNS), is essential to cognitive functions. However, when
Glu is accumulated in large concentrations at the synaptic cleft, it can induce excitotoxicity that results in secondary damage implicated in many neurological disorders. Current therapies for the treatment of neurological disorders are ineffective and have side effects associated with their use; therefore, there is a need to develop novel treatments. In this regard, previous studies have shown that neuroactive compounds obtained from the venom of the spider
have neuroprotective effects
and
In this sense, this work aimed to evaluate potential neuroprotective effects of fraction RT10, obtained from this spider venom, on primary cultures of neuron and glial cells subjected to glutamate excitotoxicity insults.
Primary cultures of neurons and glia were obtained from the cerebral tissue of 1-day-old postnatal Wistar rats. After 7 days
(DIV), the cultures were incubated with fraction RT10 (0.002; 0.02; 0.2 and 2 µg/µL) or riluzole (100 µM) for 3-hours before application of 5 mM
Glu. After 12 hours, the resazurin sodium salt (RSS) test was applied to measure metabolic activity and proliferation of living cells, whereas immunocytochemistry for MAP2 was performed to measure neuronal survival. In addition, the cells were immunolabeled with NeuN and GFAP in baseline conditions.
In the RSS tests, we observed that pre-incubation with RT10 before the excitotoxic insults from
-Glu resulted in neuroprotection, shown by a 10% reduction in the cell death level. RT10 was more effective than riluzole, which resulted in a cell-death reduction of 5%. Moreover, qualitative analysis of neuronal morphology (by MAP2 staining, expressed as fluorescence intensity (FI), an indirect measure of neuronal survival) indicate that RT10 reduced the toxic effects of
-Glu, as shown by a 38 % increase in MAP2 fluorescence when compared to
-Glu insult. On the other hand, the riluzole treatment resulted in 17% increase of MAP2 fluorescence; therefore, the neuroprotection from RT10 was more efficacious.
RT10 fraction exhibits neuroprotective effects against
-Glu excitotoxicity in neuron-glia cultured
.
Metrics
Details
- Title
- Neuroprotective properties of RT10, a fraction isolated from Parawixia bistriata spider venom, against excitotoxicity injury in neuron-glia cultures
- Creators
- Eduardo Octaviano Primini - Universidade de São PauloJosé Luiz Liberato - Universidade de São PauloAndreia Cristina Karklin Fontana - Drexel UniversityWagner Ferreira Dos Santos - Universidade de São Paulo
- Publication Details
- The journal of venomous animals and toxins including tropical diseases, v 25, pp e148818-e148818
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000468311900001
- Scopus ID
- 2-s2.0-85075563717
- Other Identifier
- 991020099147004721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Toxicology
- Tropical Medicine
- Zoology