Journal article
New methods for the detection of orthopedic and other biofilm infections
FEMS immunology and medical microbiology, v 61(2), pp 133-140
Mar 2011
PMID: 21204998
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The detection and identification of bacteria present in natural and industrial ecosystems is now entirely based on molecular systems that detect microbial RNA or DNA. Culture methods were abandoned, in the 1980s, because direct observations showed that <1% of the bacteria in these systems grew on laboratory media. Culture methods comprise the backbone of the Food and Drug Administration-approved diagnostic systems used in hospital laboratories, with some molecular methods being approved for the detection of specific pathogens that are difficult to grow in vitro. In several medical specialties, the reaction to negative cultures in cases in which overt signs of infection clearly exist has produced a spreading skepticism concerning the sensitivity and accuracy of traditional culture methods. We summarize evidence from the field of orthopedic surgery, and from other medical specialties, that support the contention that culture techniques are especially insensitive and inaccurate in the detection of chronic biofilm infections. We examine the plethora of molecular techniques that could replace cultures in the diagnosis of bacterial diseases, and we identify the new Ibis technique that is based on base ratios (not base sequences), as the molecular system most likely to fulfill the requirements of routine diagnosis in orthopedic surgery.
Metrics
Details
- Title
- New methods for the detection of orthopedic and other biofilm infections
- Creators
- John William Costerton - Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, PA, USA. wcostert@wpahs.orgJames Christopher PostGarth D EhrlichFen Z HuRachael KreftLaura NisticoSandeep KathjuPaul StoodleyLuanne Hall-StoodleyGerhard MaaleGarth JamesNick SotereanosPatrick DeMeo
- Publication Details
- FEMS immunology and medical microbiology, v 61(2), pp 133-140
- Publisher
- Wiley; England
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000287316800001
- Scopus ID
- 2-s2.0-79951514425
- Other Identifier
- 991014877912304721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Immunology
- Infectious Diseases
- Microbiology