Journal article
New vectors for epitope tagging and gene disruption in Schizosaccharomyces pombe
BioTechniques, v 55(5)
Nov 2013
PMID: 24215641
Abstract
We describe a series of new vectors for PCR-based epitope tagging and gene disruption in the fission yeast Schizosaccharomyces pombe, an exceptional model organism for the study of cellular processes. The vectors are designed for amplification of gene-targeting DNA cassettes and integration into specific genetic loci, allowing expression of proteins fused to 12 tandem copies of the Pk (V5) epitope or 5 tandem copies of the FLAG epitope with a glycine linker. These vectors are available with various antibiotic or nutritional markers and are useful for protein studies using biochemical and cell biological methods. We also describe new vectors for fluorescent protein-tagging and gene disruption using ura4MX6, LEU2MX6, and his3MX6 selection markers, allowing researchers in the S. pombe community to disrupt genes and manipulate genomic loci using primer sets already available for the widely used pFA6a-MX6 system. Our new vectors may also be useful for gene manipulation in Saccharomyces cerevisiae.
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Details
- Title
- New vectors for epitope tagging and gene disruption in Schizosaccharomyces pombe
- Creators
- Mariana C Gadaleta - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PAOsamu Iwasaki - Department of Gene Expression and Regulation, The Wistar Institute, Philadelphia, PAChiaki Noguchi - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PAKen-ichi Noma - Department of Gene Expression and Regulation, The Wistar Institute, Philadelphia, PAEishi Noguchi - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA
- Publication Details
- BioTechniques, v 55(5)
- Grant note
- R01 GM077604 || GM / National Institute of General Medical Sciences : NIGMS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000336941500008
- Scopus ID
- 2-s2.0-84887570671
- Other Identifier
- 991014878630104721
InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology