Journal article
Nitric oxide-mediated modification of the glycine binding site of the NMDA receptor during hypoxia in the cerebral cortex of the newborn piglet
Neurochemical research, v 29(2), pp 455-459
01 Feb 2004
PMID: 15002744
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
This study tested the hypothesis that cerebral hypoxia results in nitric oxide (NO)-mediated modification of the glycine-binding site of the N-methyl-D-aspartate (NMDA) receptor. Glycine binding characteristics were determined in normoxic, hypoxic, and hypoxic with 7-nitroindazole (7-NINA)-pretreated newborn piglets. The role of nitration was evaluated by determining binding characteristics in non-nitrated and in-vitro nitrated membranes. Bmax and Kd values were 30% higher in the hypoxic group than the normoxic and 7-NINA pretreated hypoxic groups. Kd values in the in-vitro normoxic nitrated membranes were similar to the non-nitrated hypoxic group. Bmax values in the in-vitro) normoxic nitrated membrane samples were 16% lower than in the non-nitrated hypoxic group. We conclude cerebral hypoxia causes modification of the glycine-binding site of the NMDA receptor and this modification of the glycine-binding site may be NO mediated. We propose that NO-mediated modification of the glycine-binding site of the NMDA receptor regulates calcium influx through its ion-channel.
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Details
- Title
- Nitric oxide-mediated modification of the glycine binding site of the NMDA receptor during hypoxia in the cerebral cortex of the newborn piglet
- Creators
- David F Sorrentino - Department of Pediatrics, Drexel University College of Medicine, St. Christopher's Hospital for Children, USAKaren I Fritz - Department of Pediatrics, Drexel University College of Medicine, St. Christopher's Hospital for Children, USASyed Hassan Haider - Department of Pediatrics, Drexel University College of Medicine, St. Christopher's Hospital for Children, USANehal Parikh - Department of Pediatrics, University of Texas, Houston, TexasMaria Delivoria Papadopoulos - Department of Pediatrics, Drexel University College of Medicine, St. Christopher's Hospital for Children, USAOm P Mishra - Department of Pediatrics, Drexel University College of Medicine, St. Christopher's Hospital for Children, USA
- Publication Details
- Neurochemical research, v 29(2), pp 455-459
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics
- Web of Science ID
- WOS:000188610000017
- Scopus ID
- 2-s2.0-1242291748
- Other Identifier
- 991019238706104721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Neurosciences