Journal article
Nitric oxide modulates the amphetamine effect on [H-3]glucose uptake in the brain of rats prenatally exposed to lead
Pharmacological reports, v 59(5), pp 601-605
01 Sep 2007
PMID: 18048962
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Glucose is the main source of energy for the central nervous system (CNS). In this study, we examined the effects of the psychostimulant amphetamine (AMPH) and the neuronal mediator nitric oxide (NO) on [H-3]glucose uptake in the brain of adult rats that had been prenatally exposed to lead. Lead [Pb(CH3COO)(2) center dot 3H(2)O; 250 ppm] was added to the drinking water of pregnant Wistar rats for the duration of pregnancy. On the day of parturition, lead was discontinued as an additive in the drinking water. Offspring remained with dams for 21 days. The control group consisted of rats that consumed water without lead. In adulthood, male offspring from both groups (lead-exposed and control) were pretreated with 7-nitroindazole (nNOS blocking agent) (10.0 mg/kg ip) or saline (1.0 ml/kg ip), 30 min before AMPH (1.0 mg/kg ip). After another 30 min, and 15 min before termination, all rats were injected with 6-[H-3]-D-glucose (500 mu Ci/kg ip). Brain specimens were taken (striatum, frontal cortex, hippocampus, and thalamus with hypothalamus, and pons with medulla oblongata) for determination of radioactivity in a liquid scintillation counter. We found that lead did not alter [3H]glucose uptake in brain regions studied (with exception of frontal cortex) but that AMPH increased [3H]glucose uptake in the striatum, frontal cortex and hippocampus, and that the AMPH effect was lessened in the hippocampus of lead-exposed rats. Moreover, the AMPH effect on [3H]glucose uptake in the frontal cortex, hippocampus, thalamus with hypothalamus and pons of control rats was potentiated by 7-NI pretreatment. Similar effect was observed in lead-intoxicated rats (striatum, frontal cortex and hippocampus). These results indicate that NO modulates AMM-induced [H-3]glucose uptake in the brain of rats prenatally exposed to lead.
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Details
- Title
- Nitric oxide modulates the amphetamine effect on [H-3]glucose uptake in the brain of rats prenatally exposed to lead
- Creators
- Przemyslaw Nowak - College Station Medical CenterMarek Swoboda - College Station Medical CenterRyszard Szkilnik - College Station Medical CenterJadwiga Josko - College Station Medical CenterLukasz Noras - College Station Medical CenterAdam Kwiecinski - College Station Medical CenterKatarzyna Sawczuk - College Station Medical CenterEwa Herba - College Station Medical CenterDorota Pojda-Wilczek - College Station Medical CenterRichard M. Kostrzewa - Tennessee State UniversityRyszard Brus - College Station Medical Center
- Publication Details
- Pharmacological reports, v 59(5), pp 601-605
- Publisher
- Polish Acad Sciences
- Number of pages
- 5
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Drexel University
- Web of Science ID
- WOS:000251789400014
- Scopus ID
- 2-s2.0-56149098165
- Other Identifier
- 991019415663604721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Pharmacology & Pharmacy