Journal article
Non-CD28 Costimulatory Molecules Present in T Cell Rafts Induce T Cell Costimulation by Enhancing the Association of TCR with Rafts
The Journal of immunology (1950), v 164(3), pp 1251-1259
01 Feb 2000
PMID: 10640738
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Abstract While CD28 functions as the major T cell costimulatory receptor, a number of other T cell molecules have also been described to induce T cell costimulation. Here, we investigated the mechanisms by which costimulatory molecules other than CD28 contribute to T cell activation. Non-CD28 costimulatory molecules such as CD5, CD9, CD2, and CD44 were present in the detergent-insoluble glycolipid-enriched (DIG) fraction/raft of the T cell surface, which is rich in TCR signaling molecules and generates a TCR signal upon recruitment of the TCR complex. Compared with CD3 ligation, coligation of CD3 and CD5 as an example of DIG-resident costimulatory molecules led to an enhanced association of CD3 and DIG. Such a DIG redistribution markedly up-regulated TCR signaling as observed by ZAP-70/LAT activation and Ca2+ influx. Disruption of DIG structure using an agent capable of altering cholesterol organization potently diminished Ca2+ mobilization induced by the coligation of CD3 and CD5. This was associated with the inhibition of the redistribution of DIG although the association of CD3 and CD5 was not affected. Thus, the DIG-resident costimulatory molecules exert their costimulatory effects by contributing to an enhanced association of TCR/CD3 and DIG.
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Details
- Title
- Non-CD28 Costimulatory Molecules Present in T Cell Rafts Induce T Cell Costimulation by Enhancing the Association of TCR with Rafts
- Creators
- Yumi Yashiro-Ohtani - The University of OsakaXu-Yu ZhouKazuhito Toyo-oka - The University of OsakaXu-Guang TaiCheung-Seog Park - The University of OsakaToshiyuki Hamaoka - The University of OsakaRyo Abe - Tokyo University of ScienceKensuke Miyake - Saga GroupHiromi Fujiwara - The University of Osaka
- Publication Details
- The Journal of immunology (1950), v 164(3), pp 1251-1259
- Publisher
- American Association of Immunologists (AAI)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000084910300016
- Scopus ID
- 2-s2.0-0034141469
- Other Identifier
- 991020099749104721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Immunology