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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Non-Equilibrium Dielectric Barrier Discharge Treatment of Mesenchymal Stem Cells: Charges and Reactive Oxygen Species Play the Major Role in Cell Death
Plasma processes and polymers, v 12(10), pp 1117-1127
01 Oct 2015
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Atmospheric pressure non-equilibrium plasmas are efficacious in killing both prokaryotic and eukaryotic cells. While the mechanism of plasma induced cell death has been thoroughly studied in prokaryotes, detailed investigation of plasma mediated eukaryotic cell death is still pending. When plasma is generated, four major components that interact with cells are produced: electric fields, radiation, charged particles, and neutral gas species. The goal of this study was to determine which of the plasma components are responsible for plasma-induced cell death by isolating and removing each from treatment. The C3H10T1/2 murine mesenchyme stem cell line was treated in six well plates, stained with Propidium Iodide to determine viability, and analyzed by image cytometry. Our results show that plasma-generated charges and reactive oxygen species are the primary contributors to cell death.
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Details
- Title
- Non-Equilibrium Dielectric Barrier Discharge Treatment of Mesenchymal Stem Cells: Charges and Reactive Oxygen Species Play the Major Role in Cell Death
- Creators
- Abraham Lin - Drexel University Drexel Plasma Institute Camden New JerseyNatalie Chernets - Thomas Jefferson UniversityJustine Han - Drexel University Drexel Plasma Institute Camden New JerseyYordano Alicea - Drexel University Drexel Plasma Institute Camden New JerseyDanil Dobrynin - Drexel UniversityGregory Fridman - Drexel University Drexel Plasma Institute Camden New JerseyTheresa A. Freeman - Thomas Jefferson UniversityAlexander Fridman - Drexel UniversityVandana Miller - Drexel University Drexel Plasma Institute Camden New Jersey
- Publication Details
- Plasma processes and polymers, v 12(10), pp 1117-1127
- Publisher
- Wiley
- Number of pages
- 11
- Grant note
- 1 R01 EB 013011-01 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- C. and J. Nyheim Plasma Institute; Microbiology Department Internal Research; Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000363731700009
- Scopus ID
- 2-s2.0-84945479039
- Other Identifier
- 991019169582304721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Physics, Applied
- Physics, Condensed Matter
- Physics, Fluids & Plasmas
- Polymer Science