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Non-Thermal Plasma Induced Immunogenic Cell Death In Pancreatic Cancer
Journal article   Peer reviewed

Non-Thermal Plasma Induced Immunogenic Cell Death In Pancreatic Cancer

Marian Khalili, Adam E. Snook, Wilbur B. Bowne, Alexander Fridman and Vandana Miller
Clinical plasma medicine, v 9, pp 17-17
Feb 2018
DOI: https://doi.org/10.1016/j.cpme.2017.12.027

Abstract

Pancreatic ductal adenocarcinoma (PDAC) is a lethal diagnosis, with a 5-year survival rate of approximately 7%. PDAC often presents late, when the only curative option, surgery, in no longer viable. Even after curative surgery in patients with resectable disease, early recurrence and disease progression often occur. Non-thermal plasma therapy represents a novel, non-invasive, immunotherapeutic approach for management of PDAC. We have previously reported plasma induced immunogenic cell death (ICD) of CT-26 colorectal cancer cells in vitro. Moreover, we have shown, that a whole-cell vaccine produced by in vitro treatment of cancer cells with plasma has resulted in protective immunity in a mouse model of colorectal cancer. Here we tested the ability of plasma to induce ICD in a radiation and multi-drug resistant human epithelial pancreatic adenocarcinoma cell line, Panc-02, with a nanosecond pulsed dielectric barrier discharge (nspDBD). We report (Figure 1) decreased cell viability in a dose-dependent fashion in vitro. Additionally, ICD, as evidenced by increased expression of Calreticulin (CRT), a damage associated molecular pattern (DAMP) protein, is shown in Table 1. Mitoxantrone (MTX) is a candidate positive control for ICD in the Panc-02 cell line with a 45-fold increase in CRT expression associated with an approximate 20-25% decrease in cell viability. Cisplatin resulted in a 2-fold increase in CRT expression and an approximate 20-25% decrease in cell viability. The cell viability results were dose-independent for both MTX and Cisplatin. Vaccination results of C57B/6 mice with plasma treated Panc-02 cells will be presented. [Display omitted] Figure 1: Plasma Induced Panc-02 cell viability via PI assay.

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