Journal article
Non-contact helium-based plasma for delivery of DNA vaccines. Enhancement of humoral and cellular immune responses
Human vaccines & immunotherapeutics, v 8(11), pp 1729-1733
01 Nov 2012
PMID: 22894954
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Non-viral in vivo administration of plasmid DNA for vaccines and immunotherapeutics has been hampered by inefficient delivery. Methods to enhance delivery such as in vivo electroporation (EP) have demonstrated effectiveness in circumventing this difficulty. However, the contact-dependent nature of EP has resulting side effects in animals and humans. Noncontact delivery methods should, in principle, overcome some of these obstacles. This report describes a helium plasma-based delivery system that enhanced humoral and cellular antigen-specific immune responses in mice against an intradermally administered HIV gp120-expressing plasmid vaccine (pJRFLgp120). The most efficient plasma delivery parameters investigated resulted in the generation of geometric mean antibody-binding titers that were 19-fold higher than plasmid delivery alone. Plasma mediated delivery of pJRFLgp120 also resulted in a 17-fold increase in the number of interferon-gamma spot-forming cells, a measure of CD8+ cytotoxic T cells, compared with non-facilitated plasmid delivery. This is the first report demonstrating the ability of this contact-independent delivery method to enhance antigen-specific immune responses against a protein generated by a DNA vaccine.
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Details
- Title
- Non-contact helium-based plasma for delivery of DNA vaccines. Enhancement of humoral and cellular immune responses
- Creators
- Richard J Connolly - Center for Molecular Delivery, University of South Florida; Tampa, FL, USA. rconnoll@usf.eduTaryn ChapmanAndrew M HoffMichele A KutzlerMark J JaroszeskiKenneth E Ugen
- Publication Details
- Human vaccines & immunotherapeutics, v 8(11), pp 1729-1733
- Publisher
- United States
- Grant note
- AI079706 / NIAID NIH HHS R21 AI079706 / NIAID NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Infectious Diseases (and HIV Medicine)
- Web of Science ID
- WOS:000311897900029
- Scopus ID
- 2-s2.0-84869860899
- Other Identifier
- 991014878346604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Immunology