Non-thermal Plasma Induces Apoptosis in Melanoma Cells via Production of Intracellular Reactive Oxygen Species

Rachel Sensenig; Sameer Kalghatgi; Ekaterina Cerchar; Gregory Fridman; Alexey Shereshevsky; Behzad Torabi; Krishna Priya Arjunan; Erica Podolsky; Alexander Fridman; Gary Friedman; Jane Azizkhan-Clifford; Ari D Brooks

doi:10.1007/s10439-010-0197-x

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Non-thermal Plasma Induces Apoptosis in Melanoma Cells via Production of Intracellular Reactive Oxygen Species

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Non-thermal Plasma Induces Apoptosis in Melanoma Cells via Production of Intracellular Reactive Oxygen Species

Rachel Sensenig, Sameer Kalghatgi, Ekaterina Cerchar, Gregory Fridman, Alexey Shereshevsky, Behzad Torabi, Krishna Priya Arjunan, Erica Podolsky, Alexander Fridman, Gary Friedman, …

Annals of biomedical engineering, v 39(2), pp 674-687

Feb 2011

DOI: https://doi.org/10.1007/s10439-010-0197-x

PMID: 21046465

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Abstract

Non-thermal plasma

Reactive oxygen species

Cancer therapy

Apoptosis

Plasma medicine

Non-thermal atmospheric pressure dielectric barrier discharge (DBD) plasma may provide a novel approach to treat malignancies via induction of apoptosis. The purpose of this study was to evaluate the potential of DBD plasma to induce apoptosis in melanoma cells. Melanoma cells were exposed to plasma at doses that did not induce necrosis, and cell viability and apoptotic activity were evaluated by Trypan blue exclusion test, Annexin-V/PI staining, caspase-3 cleavage, and TUNEL ® analysis. Trypan blue staining revealed that non-thermal plasma treatment significantly decreased the viability of cells in a dose-dependent manner 3 and 24 h after plasma treatment. Annexin-V/PI staining revealed a significant increase in apoptosis in plasma-treated cells at 24, 48, and 72 h post-treatment ( p< 0.001). Caspase-3 cleavage was observed 48 h post-plasma treatment at a dose of 15 J/cm 2 . TUNEL ® analysis of plasma-treated cells demonstrated an increase in apoptosis at 48 and 72 h post-treatment ( p< 0.001) at a dose of 15 J/cm 2 . Pre-treatment with N -acetyl-L-cysteine (NAC), an intracellular reactive oxygen species (ROS) scavenger, significantly decreased apoptosis in plasma-treated cells at 5 and 15 J/cm 2 . Plasma treatment induces apoptosis in melanoma cells through a pathway that appears to be dependent on production of intracellular ROS. DBD plasma production of intracellular ROS leads to dose-dependent DNA damage in melanoma cells, detected by γ -H2AX, which was completely abrogated by pre-treating cells with ROS scavenger, NAC. Plasma-induced DNA damage in turn may lead to the observed plasma-induced apoptosis. Since plasma is non-thermal, it may be used to selectively treat malignancies.

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Title: Non-thermal Plasma Induces Apoptosis in Melanoma Cells via Production of Intracellular Reactive Oxygen Species
Creators: Rachel Sensenig - Department of Surgery, College of Medicine, Drexel University, Philadelphia, PA 19102, USA
Sameer Kalghatgi - Electrical and Computer Engineering, Drexel University, Philadelphia, PA 19104, USA
Ekaterina Cerchar - Department of Surgery, College of Medicine, Drexel University, Philadelphia, PA 19102, USA
Gregory Fridman - School of Biomedical Engineering, Drexel University, Philadelphia, PA 19104, USA
Alexey Shereshevsky - Department of Surgery, College of Medicine, Drexel University, Philadelphia, PA 19102, USA
Behzad Torabi - Molecular Biology and Biochem, College of Medicine, Drexel University, Philadelphia, PA 19102, USA
Krishna Priya Arjunan - School of Biomedical Engineering, Drexel University, Philadelphia, PA 19104, USA
Erica Podolsky - Department of Surgery, College of Medicine, Drexel University, Philadelphia, PA 19102, USA
Alexander Fridman - Department of Mechanical Engineering and Mechanics, Drexel University, Philadelphia, PA 19104, USA
Gary Friedman - Electrical and Computer Engineering, Drexel University, Philadelphia, PA 19104, USA
Jane Azizkhan-Clifford - Molecular Biology and Biochem, College of Medicine, Drexel University, Philadelphia, PA 19102, USA
Ari D Brooks - Department of Surgery, College of Medicine, Drexel University, Philadelphia, PA 19102, USA
Publication Details: Annals of biomedical engineering, v 39(2), pp 674-687
Publisher: Springer Nature
Grant note: R01 EB013011-01 || EB / National Institute of Biomedical Imaging and Bioengineering : NIBIB
Resource Type: Journal article
Language: English
Academic Unit: Biochemistry and Molecular Biology; Electrical and Computer Engineering; Mechanical Engineering and Mechanics; Surgery
Web of Science ID: WOS:000287213300006
Scopus ID: 2-s2.0-79951550640
Other Identifier: 991014877934004721

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Collaboration types: Domestic collaboration
Web of Science research areas: Engineering, Biomedical

Non-thermal Plasma Induces Apoptosis in Melanoma Cells via Production of Intracellular Reactive Oxygen Species

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